Induction of alloreactive CD4 T cell tolerance in molecular chimeras: a possible role for regulatory T cells
| Autor: | Chaorui Tian, John Iacomini, Daron Forman, Eun-Suk Kang, Jesus Paez-Cortez |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
CD4-Positive T-Lymphocytes
Isoantigens Cell Transplantation Transgene Immunology CD1 Receptors Antigen T-Cell Bone Marrow Cells Mice Transgenic Thymus Gland Biology T-Lymphocytes Regulatory Interleukin 21 Mice Bone Marrow MHC class I medicine Immune Tolerance Immunology and Allergy Cytotoxic T cell Animals Transplantation Homologous IL-2 receptor Cells Cultured Antigen Presentation Chimera T-cell receptor Models Immunological Cell Differentiation Cell biology Survival Rate medicine.anatomical_structure biology.protein Female Bone marrow Gene Deletion |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 176(6) |
| ISSN: | 0022-1767 |
| Popis: | Induction of molecular chimerism following reconstitution of mice with autologous bone marrow cells expressing a retrovirally encoded allogeneic MHC class I Ag results in donor-specific tolerance. To investigate the mechanism by which CD4 T cells that recognize allogeneic MHC class I through the indirect pathway of Ag presentation are rendered tolerant in molecular chimeras, transgenic mice expressing a TCR on CD4 T cells specific for peptides derived from Kb were used. CD4 T cells expressing the transgenic TCR were detected in mice reconstituted with bone marrow cells transduced with retroviruses carrying the gene encoding H-2Kb, albeit detection was at lower levels than in mice receiving mock-transduced bone marrow. Despite the presence of CD4 T cells expressing an alloreactive TCR, mice receiving H-2Kb-transduced bone marrow permanently accepted Kb disparate skin grafts. CD4+CD25+ T cells from mice reconstituted with H-2Kb-transduced bone marrow prevented rejection of Kb disparate skin grafts when adoptively transferred into immunodeficient mice along with effector T cells, suggesting that induction of molecular chimerism leads to the generation of donor specific regulatory T cells, which may be involved in preventing alloreactive CD4 T cell responses that lead to rejection. |
| Databáze: | OpenAIRE |
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