Novel subtype C human immunodeficiency virus type 1 envelopes cloned directly from plasma: coreceptor usage and neutralization phenotypes
| Autor: | W. W. L. Koh, A. Forsman, S. Hue, G. J. van der Velden, D. L. Yirrell, A. McKnight, R. A. Weiss, M. M. I. Aasa-Chapman |
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| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Glycosylation HIV Antigens Receptors CCR3 Population HIV Infections Disease Genes env Receptors HIV Nursing Acquired immunodeficiency syndrome (AIDS) Neutralization Tests Virology medicine Childbirth Humans Cloning Molecular education Phylogeny education.field_of_study business.industry Public health env Gene Products Human Immunodeficiency Virus medicine.disease Scotland Family planning Family medicine Africa Host-Pathogen Interactions HIV-1 Health education business Qualitative research |
| Zdroj: | The Journal of general virology. 91(Pt 9) |
| ISSN: | 1465-2099 |
| Popis: | Human immunodeficiency virus type 1 (HIV-1) is classified into different phylogenetic subtypes, with subtype C representing more than half of the novel infections globally. However, there are relatively few subtype C envelopes available for study. We amplified 18 unique env genes from 13 patients who were infected with subtype C HIV-1 in six African countries and in Scotland to create replication-competent viruses. These envelopes are phylogenetically diverse across the subtype C spectrum, and have on average more N-linked glycosylation sites and slightly longer variable loops than previously described C envelopes. We found that CCR3 coreceptor usage is less prevalent in subtype C than in subtype B viruses, and these envelopes have varied sensitivity to neutralization. The subtype C chimeric viruses generated in this study will be useful for evaluating the breadth of neutralizing antibodies and other entry inhibitors. |
| Databáze: | OpenAIRE |
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