High fidelity neuronal networks formed by plasma masking with a bilayer membrane: analysis of neurodegenerative and neuroprotective processes
| Autor: | Ulrich Marggraf, Christoph van Thriel, Jean-Philippe Frimat, Jan G. Hengstler, Amir Ali Taghipour-Anvari, Evelyn Drabiniok, Heike Hardelauf, Peter Jacob, Andreas Neyer, Jonathan West, Julia Sisnaiske |
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| Rok vydání: | 2011 |
| Předmět: |
Masking (art)
Neurite Surface Properties Lipid Bilayers Biomedical Engineering Bioengineering Nanotechnology 02 engineering and technology Biochemistry Neuroprotection 03 medical and health sciences chemistry.chemical_compound Humans Dimethylpolysiloxanes 030304 developmental biology Brain-derived neurotrophic factor Acrylamide 0303 health sciences METIS-281653 Chemistry Brain-Derived Neurotrophic Factor Bilayer IR-78993 Cell Differentiation Membranes Artificial Dipeptides General Chemistry 021001 nanoscience & nanotechnology EWI-21019 Neuroprotective Agents Membrane Nerve Degeneration Biophysics Adsorption Nerve Net 0210 nano-technology Ethylene glycol Protein adsorption |
| Zdroj: | Lab on a Chip; Vol 11 Lab on a chip, 11(16), 2763-2771. Royal Society of Chemistry Lab on a Chip |
| ISSN: | 1473-0189 1473-0197 |
| DOI: | 10.1039/c1lc20257j |
| Popis: | Spatially defined neuronal networks have great potential to be used in a wide spectrum of neurobiology assays. We present an original technique for the precise and reproducible formation of neuronal networks. A PDMS membrane comprising through-holes aligned with interconnecting microchannels was used during oxygen plasma etching to dry mask a protein rejecting poly(ethylene glycol) (PEG) adlayer. Patterns were faithfully replicated to produce an oxidized interconnected array pattern which supported protein adsorption. Differentiated human SH-SY5Y neuron-like cells adhered to the array nodes with the micron-scale interconnecting tracks guiding neurite outgrowth to produce neuronal connections and establish a network. A 2.0 μm track width was optimal for high-level network formation and node compliance. These spatially standardized neuronal networks were used to analyse the dynamics of acrylamide-induced neurite degeneration and the protective effects of co-treatment with calpeptin or brain derived neurotrophic factor (BDNF). |
| Databáze: | OpenAIRE |
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