Display of the VP1 epitope of foot-and-mouth disease virus on bacteriophage T7 and its application in diagnosis
| Autor: | Wen Siang Tan, Chuan Loo Wong, Chin Chin Sieo |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Phage display
T7 phage medicine.drug_class viruses Cattle Diseases Enzyme-Linked Immunosorbent Assay Antibodies Viral Monoclonal antibody Virus Epitope Bacteriophage Epitopes Bacteriophage T7 Virology medicine Animals Antigens Viral biology biology.organism_classification Recombinant Proteins Capsid Foot-and-Mouth Disease Virus Foot-and-Mouth Disease Capsid Proteins Cattle Foot-and-mouth disease virus Cell Surface Display Techniques |
| Zdroj: | Journal of Virological Methods. 193:611-619 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/j.jviromet.2013.07.053 |
| Popis: | Foot-and-mouth disease (FMD) is a highly contagious epidemic disease threatening the cattle industry since the sixteenth century. In recent years, the development of diagnostic assays for FMD has benefited considerably from the advances of recombinant DNA technology. In this study, the immunodominant region of the capsid protein VP1 of the foot-and-mouth disease virus (FMDV) was fused to the T7 bacteriophage and expressed on the surface of the bacteriophage capsid protein. The recombinant protein of about 42 kDa was detected by the anti-T7 tag monoclonal antibody in Western blot analysis. Phage ELISA showed that both the vaccinated and positive infected bovine sera reacted significantly with the recombinant T7 particle. This study demonstrated the potential of the T7 phage displaying the VP1 epitope as a diagnostic reagent. |
| Databáze: | OpenAIRE |
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