Optimization of nitrogen feeding strategies for improving polyhydroxybutyrate production from biogas by Methylocystis parvus str. OBBP in a stirred tank reactor

Autor: Rodríguez Muñoz, Yadira, García, Silvia María, Pérez Fernández, Rebeca, Lebrero Fernández, Raquel, Muñoz Torre, Raúl
Rok vydání: 2021
Předmět:
Zdroj: UVaDOC. Repositorio Documental de la Universidad de Valladolid
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ISSN: 1879-1298
2016-0771
Popis: Producción Científica
The design of efficient cultivation strategies to produce bioplastics from biogas is crucial for the implementation of this biorefinery process. In this work, biogas-based polyhydroxybutyrate (PHB) production and CH4 biodegradation performance was investigated for the first time in a stirred tank bioreactor inoculated with Methylocystis parvus OBBP. Decreasing nitrogen loading rates in continuous mode and alternating feast:famine regimes of 24 h-cycles, and alternating feast:famine regimes of 24 h:24 h and 24 h:48 h were tested. Continuous N feeding did not support an effective PHB production despite the occurrence of nitrogen limiting conditions. Feast-famine cycles of 24 h:24 h (with 50% stoichiometric nitrogen supply) supported the maximum PHB production (20 g-PHB m−3 d−1) without compromising the CH4-elimination capacity (25 g m−3 h−1) of the system. Feast:famine ratios ≤1:2 entailed the deterioration of process performance at stoichiometric nitrogen inputs ≤60%.
Ministerio de Ciencia, Innovación y Universidades (grant BES-2016-077160)
Ministerio de Ciencia, Innovación y Universidades (project CTM2015-70442-R)
Junta de Castilla y León (projects UIC 071 and CLU 2017–09)
Databáze: OpenAIRE
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