Species identification by the positional analysis of fatty acid composition in triacylglyceride of adipose and bone tissues
| Autor: | Masato Kagawa, Kazuo Matsubara, Hiroshi Shiono, Kojiro Kimura, Yuko Fukui |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adult
Chromatography Gas Swine Adipose tissue Biology Positional analysis Bone and Bones Pathology and Forensic Medicine chemistry.chemical_compound Dogs Species Specificity Species identification Animals Humans Triglycerides chemistry.chemical_classification Degree of unsaturation Triglyceride Fatty Acids Fatty acid Forensic Medicine Middle Aged Biochemistry chemistry Adipose Tissue Cats Composition (visual arts) Cattle Fatty acid composition Law Chickens |
| Zdroj: | Forensic science international. 79(3) |
| ISSN: | 0379-0738 |
| Popis: | In a new attempt at species identification, the total composition and positional distribution of fatty acid in triacylglyceride (TG) of adipose and bone tissues were analyzed in human, bovine, pig, dog, cat and chicken tissues. Although the total fatty acid compositions of bovine and pig tissues were significantly different from those of human (different in more than half the fatty acids tested), dog, cat and chicken tissues showed a comparatively similar composition to human fatty acids composed of TG in both tissues. The TG in these tissues was also subjected to stereospecific analysis using pancreatic lipase, that is, the fatty acid distribution in positions 1,3 (not distinguished between 1 and 3) and 2 of the TG were determined. The distribution of fatty acids among the positions 1,3 and 2 in the TG of animal adipose and bone tissues was non-random. The distribution between position 2 and positions 1,3 seems to be governed by chain length and unsaturation in each animal. The shorter and more unsaturated fatty acids showed a greater tendency to occupy position 2 of TG. Although this rule appeared in all animals except the pig, the distribution of each fatty acid into position 2 was species-specific. The positional distribution of fatty acid in TG was identical among the same species and in different regions of the same body. Thus, even when species identification is difficult using the pattern of total fatty acid composition, the analysis of the positional distribution of fatty acid makes it possible to determine the species. From the present results, the evaluation of positional distribution of fatty acid in the TG is a useful tool for the identification of human tissues. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect