Dual Recombining-out System for Spatiotemporal Gene Expression in C. elegans
| Autor: | Rong Li, Umar Al-Sheikh, Sheng-Wu Yin, Zheng-Xing Wu, Jing-Jing Wu, Ming-Hai Ge, Ping-Zhou Wang, Tai-Hong Wu, Wei Wang, Hui Liu, Jia-Hao Huang, Xin Wen, Qing-Qin He, Li-Li Chen |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell type Multidisciplinary Cell Promoter 02 engineering and technology Biology 021001 nanoscience & nanotechnology Article Cell biology 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Spatiotemporal gene expression Transcription (biology) Gene expression medicine Recombinase lcsh:Q lcsh:Science Genetic Engineering 0210 nano-technology Molecular Biology Gene Neuroscience |
| Zdroj: | iScience iScience, Vol 23, Iss 10, Pp 101567-(2020) |
| ISSN: | 2589-0042 |
| DOI: | 10.1016/j.isci.2020.101567 |
| Popis: | Summary Specific recording, labeling, and spatiotemporal manipulating neurons are essential for neuroscience research. In this study, we developed a tripartite spatiotemporal gene induction system in C. elegans, which is based on the knockout of two transcriptional terminators (stops in short) by two different recombinases FLP and CRE. The recombinase sites (loxP and FRT) flanked stops after a ubiquitous promoter terminate transcription of target genes. FLP and CRE, induced by two promoters of overlapping expression, remove the stops (subsequent FLP/CRE-out). The system provides an "AND" gate strategy for specific gene expression in single types of cell(s). Combined with an inducible promoter or element, the system can control the spatiotemporal expression of genes in defined cell types, especially in cells or tissues lacking a specific promoter. This tripartite FLP/CRE-out gene expression system is a simple, labor- and cost-saving toolbox for cell type-specific and inducible gene expression in C. elegans. Graphical Abstract Highlights • A tripartite FLP/CRE-out spatiotemporal gene expression system in C. elegans • Eight promoter pairs with the targeting of a single-type neuron(s) were identified • The system is easy to use and cost and labor saving Molecular Biology; Neuroscience; Genetic Engineering |
| Databáze: | OpenAIRE |
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