Quantification of aminobutyric acids and their clinical applications as biomarkers for osteoporosis

Autor: Kuan Jui Su, Chenglin Mo, Maciej Kukula, Daniel W. Armstrong, Robert R. Recker, Zhiying Wang, Joan M. Lappe, Marco Brotto, Lan Juan Zhao, Jauh Tzuoh Lee, Hui Shen, Lynda F. Bonewald, Liangqiao Bian, Hong-Wen Deng
Rok vydání: 2020
Předmět:
0301 basic medicine
Osteoporosis
Medicine (miscellaneous)
Disease
Mice
0302 clinical medicine
Tandem Mass Spectrometry
lcsh:QH301-705.5
Aged
80 and over
Bone mineral
Aminobutyrates
Middle Aged
Prognosis
Body Fluids
3. Good health
Metabolome
Female
General Agricultural and Biological Sciences
Signal Transduction
musculoskeletal diseases
medicine.medical_specialty
030209 endocrinology & metabolism
Single-nucleotide polymorphism
Predictive markers
Models
Biological
Sensitivity and Specificity
Aminobutyric acid
Article
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Internal medicine
medicine
Dihydropyrimidine dehydrogenase
Animals
Humans
Metabolomics
Aged
business.industry
Gene Expression Profiling
Reproducibility of Results
medicine.disease
Peripheral blood
Osteopenia
030104 developmental biology
Endocrinology
lcsh:Biology (General)
business
Biomarkers
Chromatography
Liquid
Zdroj: Communications Biology
Communications Biology, Vol 3, Iss 1, Pp 1-14 (2020)
ISSN: 2399-3642
DOI: 10.1038/s42003-020-0766-y
Popis: Osteoporosis is a highly prevalent chronic aging-related disease that frequently is only detected after fracture. We hypothesized that aminobutyric acids could serve as biomarkers for osteoporosis. We developed a quick, accurate, and sensitive screening method for aminobutyric acid isomers and enantiomers yielding correlations with bone mineral density (BMD) and osteoporotic fracture. In serum, γ-aminobutyric acid (GABA) and (R)-3-aminoisobutyric acid (D-BAIBA) have positive associations with physical activity in young lean women. D-BAIBA positively associated with hip BMD in older individuals without osteoporosis/osteopenia. Lower levels of GABA were observed in 60–80 year old women with osteoporotic fractures. Single nucleotide polymorphisms in seven genes related to these metabolites associated with BMD and osteoporosis. In peripheral blood monocytes, dihydropyrimidine dehydrogenase, an enzyme essential to D-BAIBA generation, exhibited positive association with physical activity and hip BMD. Along with their signaling roles, BAIBA and GABA might serve as biomarkers for diagnosis and treatments of osteoporosis.
Wang et al. develop an LC/MS based screening method to separate and quantify aminobutyric acids isoforms. Applying it to osteoporosis clinical studies, their method yields important correlations with bone mineral density and osteoporotic fracture and highlight the role of γ-aminobutyric acid and β-aminoisobutyric acid as biomarkers for osteoporosis.
Databáze: OpenAIRE
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