Effects of elevated temperature on gonadal functions, cellular apoptosis, and oxidative stress in Atlantic sea urchin Arbacia punculata
| Autor: | Sarah Nash, Jackson Johnstone, Saydur Rahman, Eleazar Hernandez |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
0106 biological sciences Hot Temperature Gonad media_common.quotation_subject Apoptosis Aquatic Science Oceanography medicine.disease_cause Global Warming 010603 evolutionary biology 01 natural sciences Protein Carbonylation Andrology chemistry.chemical_compound biology.animal medicine Animals HSP70 Heat-Shock Proteins Seawater Gonads Sea urchin media_common Arbacia Environmental Biomarkers biology urogenital system Chemistry Reproduction 010604 marine biology & hydrobiology Nitrotyrosine General Medicine Hydrogen-Ion Concentration biology.organism_classification Reactive Nitrogen Species Pollution Sperm Hsp70 Oxidative Stress medicine.anatomical_structure Female Reactive Oxygen Species Oxidative stress |
| Zdroj: | Marine Environmental Research. 149:40-49 |
| ISSN: | 0141-1136 |
| DOI: | 10.1016/j.marenvres.2019.05.017 |
| Popis: | Increasing seawater temperature affects growth, reproduction and development in marine organisms. In this study, we examined the effects of elevated temperatures on reproductive functions, heat shock protein 70 (HSP70) and nitrotyrosine protein (NTP, an indicator of reactive nitrogen species) expressions, protein carbonyl (PC, an indicator of oxidative stress) contents, cellular apoptosis, and coelomic fluid (CF) conditions in Atlantic sea urchin. Sea urchins were housed in six aquaria with control (24 °C) and elevated temperatures (28 °C and 32 °C) for a 7-day period. After exposure, sea urchins exhibited decreased percentages of gametes (eggs/sperm), as well as increased HSP70 and NTP expressions in eggs and spermatogenic cells, increased gonadal apoptosis, and decreased CF pH compared to controls. PC contents were also significantly increased in gonadal tissues at higher temperatures. These results suggest that elevated temperature acidifies CF, increases oxidative stress and gonadal apoptosis, and results in impairment of reproductive functions in sea urchins. |
| Databáze: | OpenAIRE |
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