β-Amyloid-induced activation of Caspase-3 in primary cultures of rat neurons
| Autor: | Marta Llansola, Nuria Marín, Francisco Bosch-Morell, Belén Romero, Francisco J. Romero, Vicente Felipo, Joaquín Romá |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Aging
Time Factors Amyloid Proteolysis Apoptosis Caspase 3 medicine Animals Cells Cultured Caspase Neurons Amyloid beta-Peptides biology medicine.diagnostic_test Neurodegeneration Intrinsic apoptosis medicine.disease Molecular biology Peptide Fragments Rats Enzyme Activation medicine.anatomical_structure Caspases Immunology biology.protein Neuron Developmental Biology |
| Zdroj: | Mechanisms of Ageing and Development. 119:63-67 |
| ISSN: | 0047-6374 |
| DOI: | 10.1016/s0047-6374(00)00172-x |
| Popis: | It is known that beta-amyloid peptide (Abeta) contributes to the neurodegeneration in Alzheimer's disease (AD) and operates through activation of an apoptotic pathway. Apoptotic signal is driven by a family of cysteine proteases called caspases. The beta-amyloid precursor protein (APP) is directly and efficiently cleaved by caspases during apoptosis, resulting in elevated beta-amyloid peptide formation. Cerebellar neurons from rat pups were treated with the aged Abeta(25-35) at 1 and 5 microM and fluorescence assays of caspase activity performed over 4 days. We observed an increase in caspase activity after 48 h treatment in both 1 and 5 microM treated cells, then (72-96 h) caspase activity decreased to control values. The data presented support the hypothesis that Abeta(25-35)-induced apoptosis is mediated by the activation of Caspase-3 and that this is a transient effect. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect