Phytoplankton trigger the production of cryptic metabolites in the marine actinobacterium Salinispora tropica
| Autor: | Joseph Alexander Christie-Oleza, Despoina Sousoni, Maria del Mar Aguilo-Ferretjans, Audam Chhun, Lijiang Song, Christophe Corre |
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| Přispěvatelé: | Natural Environment Research Council (UK), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Biotechnology and Biological Sciences Research Council (UK), Engineering and Physical Sciences Research Council (UK) |
| Rok vydání: | 2021 |
| Předmět: |
lcsh:Biotechnology
Physics::Optics Bioengineering Computational biology Proteomics Applied Microbiology and Biotechnology Biochemistry Computer Science::Digital Libraries Actinobacteria 03 medical and health sciences Metabolomics lcsh:TP248.13-248.65 Condensed Matter::Superconductivity Phytoplankton parasitic diseases 14. Life underwater Gene Research Articles 030304 developmental biology 0303 health sciences biology Phototroph 030306 microbiology QH Computer Science::Information Retrieval QK fungi Micromonosporaceae Computer Science::Social and Information Networks biology.organism_classification Phylum Actinobacteria Salinispora tropica Multigene Family Biotechnology Research Article |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Microbial Biotechnology Microbial Biotechnology, Vol 14, Iss 1, Pp 291-306 (2021) |
| ISSN: | 1751-7907 |
| Popis: | Thematic Issue: Crystal Ball. Filamentous members of the phylum Actinobacteria are a remarkable source of natural products with pharmaceutical potential. The discovery of novel molecules from these organisms is, however, hindered because most of the biosynthetic gene clusters (BGCs) encoding these secondary metabolites are cryptic or silent and are referred to as orphan BGCs. While co-culture has proven to be a promising approach to unlock the biosynthetic potential of many microorganisms by activating the expression of these orphan BGCs, it still remains an underexplored technique. The marine actinobacterium Salinispora tropica, for instance, produces valuable compounds such as the anti-cancer molecule salinosporamide but half of its putative BGCs are still orphan. Although previous studies have used marine heterotrophs to induce orphan BGCs in Salinispora, its co-culture with marine phototrophs has yet to be investigated. Following the observation of an antimicrobial activity against a range of phytoplankton by S. tropica, we here report that the photosynthate released by photosynthetic primary producers influences its biosynthetic capacities with production of cryptic molecules and the activation of orphan BGCs. Our work, using an approach combining metabolomics and proteomics, pioneers the use of phototrophs as a promising strategy to accelerate the discovery of novel natural products from marine actinobacteria. A.C. was supported by an MIBTP PhD scholarship (BB/M01116X/1) and D.S. by a NERC CENTA DTP studentship (NE/L002493/1). J.A.C.-O was funded by a NERC Independent Research Fellowship NE/K009044/1 and Ramón y Cajal contract RYC-2017-22452 (funded by the Ministry of Science, Innovation and Universities, the National Agency of Research, and the European Social Fund). C.C. thanks BBSRC (grant BB/M022765/1) and European Union’s Horizon 2020 research No. 765147 for support. L.S. would like to acknowledge BBSRC (BB/M017982/1 and BB/R000689/1) and EPSRC (EP/P0305721/1) for financial support. |
| Databáze: | OpenAIRE |
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