Effect of gene disruption of succinate dehydrogenase on succinate production in a sake yeast strain
| Autor: | Shigeru Nakamori, Yoshito Kubo, Hiroshi Takagi |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
chemistry.chemical_classification
Strain (chemistry) Succinate dehydrogenase Saccharomyces cerevisiae Bioengineering macromolecular substances Biology biology.organism_classification Applied Microbiology and Biotechnology Yeast chemistry.chemical_compound Enzyme Biochemistry chemistry Succinic acid Glycerol biology.protein Biotechnology Organic acid |
| Zdroj: | Journal of bioscience and bioengineering. 90(6) |
| ISSN: | 1389-1723 |
| Popis: | Succinate dehydrogenase (SDH) of Saccharomyces cerevisiae consists of four subunits encoded by the SDH1, SDH2, SDH3, and SDH4 genes. We determined the effect of SDH deficiency on the productivity of organic acids in a sake yeast strain Kyokai no. 9. The SDH activity of single disruptants was retained at 30-90% of that of the wild-type strain, but the activity disappeared in double disruptants of the SDH1 and SDH2 or SDH1b (the SDH1 homologue) genes. Two double disruptants showed no growth on a medium containing glycerol as the sole carbon source, while the single disruptants could utilize glycerol. These results indicate that double disruption of the SDH1 and SDH2 or SDH1b genes is required for complete loss of SDH activity and that the SDH1b gene compensates for the function of the SDH1 gene. The sdh1 sdh1b disruptant showed a marked increase in succinate productivity of up to 1.9-fold along with a decrease in malate productivity relative to the wild-type strains under shaking conditions. Under both static and sake brewing conditions, the productivity of these organic acids in the disruptants was virtually unchanged from that in the wild-type strain. Furthermore, SDH activity was undetectable in the wild-type and the disrupted strains under static conditions. These results suggest that SDH activity contributes to succinate production under shaking conditions, but not under static and sake brewing conditions. |
| Databáze: | OpenAIRE |
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