The effect of different vitrification protocols on cell survival in human ovarian tissue: a pilot study
| Autor: | Sabine Dekan, Julian Marschalek, Christian Egarter, J Ott, Detlef Pietrowski, Kazem Nouri, Maria Frank |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Cell viability Adolescent Cell Survival Pilot Projects Biology Young Adult Text mining Humans Dimethyl Sulfoxide Vitrification Fertility preservation Cell survival Cryopreservation business.industry Ovarian tissue Research Ovary Ovarian tissue cryopreservation Obstetrics and Gynecology Gynecology and obstetrics Oncology RG1-991 Cancer research Transgender Female business |
| Zdroj: | Journal of Ovarian Research Journal of Ovarian Research, Vol 14, Iss 1, Pp 1-11 (2021) |
| ISSN: | 1757-2215 |
| DOI: | 10.1186/s13048-021-00924-8 |
| Popis: | Background Vitrification has superseded the slow freezing method for cryopreservation of oocytes, embryos, and sperm, but there are as yet no standard protocols for its use in ovarian tissue cryopreservation (OTC). Published protocols diverge mainly with regard to the extent of supplementation of dimethyl sulfoxide (DMSO) to the vitrification medium, and to the use of an open or closed vitrification system. We investigated the viability of cells after vitrification/warming, using ovarian tissue of transgender patients, by means of Fluorescence Activated Cells Sorting (FACS), and histomorphological analyses using a DMSO-containing (P1) and a DMSO-free protocol (P2) in an open or closed vitrification setting. Results Twelve ovarian samples were donated from female-to-male transgender patients: 6 were vitrified according to protocol 1, the other 6 according to protocol 2. The amount of viable cells was 90.1% (P1) and 88.4% (P2) before vitrification. After vitrification and subsequent warming, viable cells were reduced to 82.9% (P1, p = 0.093) and 72.4% (P2, p = 0.019). When comparing the closed and the open systems, the decline in cell viability from pre- to post-vitrification was significant only for the latter (p = 0.037). Histological examination reveals no significant differences with respect to degenerated follicles before or after vitrification. Conclusion These results led us to conclude that a protocol containing DMSO results in a higher viability of ovarian cells than a protocol that uses ethylene glycol as cryoprotective agent in vitrification. The use of an open vitrification system led to significant decline in the rate of viable cells. Trial registration NCT03649087, retrospectively registered 28.08.2018. |
| Databáze: | OpenAIRE |
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