Generation of transgenic plants expressing plasma membrane-bound antibodies to the environmental pollutant microcystin-LR
| Autor: | Craig J. van Dolleweerd, Matthew Ross Drever, Tommaso Barbi, Julian K.-C. Ma, Pascal M. W. Drake, Andrew R. Porter |
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| Rok vydání: | 2010 |
| Předmět: |
Microcystins
medicine.drug_class Transgene Enzyme-Linked Immunosorbent Assay Microcystin-LR Monoclonal antibody medicine.disease_cause Antibodies law.invention chemistry.chemical_compound law Tobacco Botany Genetics medicine biology Toxin Cell Membrane Plants Genetically Modified Molecular biology Recombinant Proteins Plant Leaves Transformation (genetics) chemistry Recombinant DNA biology.protein Environmental Pollutants Marine Toxins Animal Science and Zoology Antibody Agronomy and Crop Science Marine toxin Biotechnology |
| Zdroj: | Transgenic Research. 20:701-707 |
| ISSN: | 1573-9368 0962-8819 |
| DOI: | 10.1007/s11248-010-9431-4 |
| Popis: | In this paper we describe the engineering and regeneration of transgenic tobacco plants expressing a recombinant plasma membrane-retained antibody specific to microcystin-LR (MC-LR), the environmental toxin pollutant produced by cyanobacteria. The antibody was created by a genetic fusion of the antigen binding regions of the microcystin-specific single chain antibody, 3A8, with the constant regions from the murine IgG1κ, Guy's 13, including a membrane retention sequence at the C-terminal end of the antibody heavy chain. The antibody produced in the leaves was shown to be functional by binding to MC-LR in an ELISA with antibody yields in transgenic plant leaves reaching a maximum of 1.2 μg g(-1) leaf f.wt (0.005% total soluble protein). Antibody-MC-LR complexes formed in leaves after addition of MC-LR to hydroponic medium around the roots of transgenic plant cultures. |
| Databáze: | OpenAIRE |
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