Amplification-free detection of SARS-CoV-2 using gold nanotriangles functionalized with oligonucleotides

Autor: Rafael del Caño, Tania García-Mendiola, Daniel García-Nieto, Raquel Álvaro, Mónica Luna, Hernán Alarcón Iniesta, Rocío Coloma, Ciro Rodríguez Diaz, Paula Milán-Rois, Milagros Castellanos, Melanie Abreu, Rafael Cantón, Juan Carlos Galán, Teresa Pineda, Félix Pariente, Rodolfo Miranda, Álvaro Somoza, Encarnación Lorenzo
Přispěvatelé: Consejo Superior de Investigaciones Científicas (España), Conferencia de Rectores de las Universidades Españolas, Comunidad de Madrid, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), European Commission, Banco Santander, Instituto de Salud Carlos III, UAM. Departamento de Química Analítica y Análisis Instrumental
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Repositorio Institucional del Instituto Madrileño de Estudios Avanzados en Nanociencia
instname
Digital.CSIC. Repositorio Institucional del CSIC
Biblos-e Archivo. Repositorio Institucional de la UAM
Universidad Camilo José Cela (UCJC)
Popis: Gold nanotriangles (AuNTs) functionalized with dithiolated oligonucleotides have been employed to develop an amplification-free electrochemical biosensor for SARS-CoV-2 in patient samples. Gold nanotriangles, prepared through a seed-mediated growth method and exhaustively characterized by different techniques, serve as an improved electrochemical platform and for DNA probe immobilization. Azure A is used as an electrochemical indicator of the hybridization event. The biosensor detects either single stranded DNA or RNA sequences of SARS-CoV-2 of different lengths, with a low detection limit of 22.2 fM. In addition, it allows to detect point mutations in SARS-CoV-2 genome with the aim to detect more infective SARS-CoV-2 variants such as Alpha, Beta, Gamma, Delta, and Omicron. Results obtained with the biosensor in nasopharyngeal swab samples from COVID-19 patients show the possibility to clearly discriminate between non-infected and infected patient samples as well as patient samples with different viral load. Furthermore, the results correlate well with those obtained by the gold standard technique RT-qPCR, with the advantage of avoiding the amplification process and the need of sophisticated equipment.
Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This work is funded by the Community of Madrid (TRANSNANOAVANSENS S2018/NMT-4349), Spanish Ministry of Economy and Competitiveness (PID2020-116728RB-I00, CTQ2015-71955-RED ELECTROBIONET), and the Community of Madrid (project S2018/NMT-4291 TEC2SPACE), Spanish Ministry of Economy and Competitiveness (project CSIC13-4E-1794) and EU (FEDER, FSE). RdC received grants from UAM, Banco Santander, Fundación IMDEA (convocatoria CRUE-CSIC-SANTANDER, fondo supera 2020, project with reference 10.01.03.02.41). JCG received grants from Ramón y Cajal Institute for Health Research (IRYCIS), Madrid, Spain (COVID-19 Grant, 2020/0154). This research was also funded by the Spanish Ministry of Economy and Competitiveness (SAF2017-87305-R, PID2020-119352RB-I00), Instituto de Salud Carlos III (FONDO-COVID19: COV20/00144 and COV20/00122). P.M.R received grants from the Ministry of Economy, Industry and competitiveness of Spain (FPI grant (BES-2017.082521)). IMDEA Nanociencia received grants from the “Severo Ochoa” Programme for Centres of Excellence in R&D (MINECO, Grant SEV-2016–0686, CEX2020-001039-S).
Databáze: OpenAIRE
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