TILRR, a Novel IL-1RI Co-receptor, Potentiates MyD88 Recruitment to Control Ras-dependent Amplification of NF-κB*
| Autor: | Endre Kiss-Toth, Eva E. Qwarnstrom, Ian R. Palmer, G. J. S. Fowler, Xiao Zhang, Selina McHarg, Hong B. Kim, Freya Shephard, Luke A. J. O'Neill |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Receptor complex
NF-kappaB Signaling Receptor expression Molecular Sequence Data Cytoskeleton/Actin GTPase Biology Biochemistry Cell Line Mice Animals Humans Signal Transduction/Adapter Proteins Amino Acid Sequence RNA Small Interfering Receptor Transcription factor Molecular Biology Genetics Interleukin-8 NF-kappa B Receptors Interleukin-1 Cell Biology Receptors Interleukin Receptor Complex Formation and Stoichiometry Cell biology Receptors/Cytokine Enzyme Activation Interleukin-1 Receptor Type 1 Proteoglycans Structure Gene Expression Regulation Mutagenesis Myeloid Differentiation Factor 88 ras Proteins Additions and Corrections Signal transduction Sequence Alignment Signal Transduction Receptors/Structure-Function Interleukin-1 |
| Zdroj: | The Journal of Biological Chemistry |
| ISSN: | 1083-351X 0021-9258 |
| Popis: | Host defense against infection is induced by Toll-like and interleukin (IL)-1 receptors, and controlled by the transcription factor NF-kappaB. Our earlier studies have shown that IL-1 activation impacts cytoskeletal structure and that IL-1 receptor (IL-1RI) function is substrate-dependent. Here we identify a novel regulatory component, TILRR, which amplifies activation of IL-1RI and coordinates IL-1-induced control with mechanotransduction. We show that TILRR is a highly conserved and widely expressed enhancer of IL-1-regulated inflammatory responses and, further, that it is a membrane-bound glycosylated protein with sequence homology to members of the FRAS-1 family. We demonstrate that TILRR is recruited to the IL-1 receptor complex and magnifies signal amplification by increasing receptor expression and ligand binding. In addition, we show that the consequent potentiation of NF-kappaB is controlled through IL-1RI-associated signaling components in coordination with activation of the Ras GTPase. Using mutagenesis, we demonstrate that TILRR function is dependent on association with its signaling partner and, further, that formation of the TILRR-containing IL-1RI complex imparts enhanced association of the MyD88 adapter during ligand-induced activation of NF-kappaB. We conclude that TILRR is an IL-1RI co-receptor, which associates with the signaling receptor complex to enhance recruitment of MyD88 and control Ras-dependent amplification of NF-kappaB and inflammatory responses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|