A simple approach to optimum pool size for pooled SARS-CoV-2 testing
| Autor: | Regen, Francesca, Eren, Neriman, Heuser, Isabella, Hellmann-Regen, Julian |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
SARS-CoV-2 Data Collection Testing capacity RT-qPCR SARS-VoV-2 COVID-19 General Medicine Pool size Article lcsh:Infectious and parasitic diseases Infectious Diseases COVID-19 Testing Molecular Diagnostic Techniques Humans lcsh:RC109-216 Sample pooling Pandemics 600 Technik Medizin angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit |
| Zdroj: | International Journal of Infectious Diseases, Vol 100, Iss, Pp 324-326 (2020) International Journal of Infectious Diseases |
| ISSN: | 1201-9712 |
| Popis: | Highlights • A guide towards rational decision on sample pooling / pool size • Sample pooling in SARS-CoV-2 RT-qPCR strikingly increases throughput • Pool size strikingly depends on target prevalence rates • While cost-savings from pooling – and optimum pool sizes – have been largely unclear, we present a simple-to-use formula to calculate the optimum pool size • Our results are applicable / generalizable to non-SARS-related testing strategies as well Systematic, large-scale testing of asymptomatic subjects is an important strategy in the management of the SARS-CoV-2 pandemic. In order to increase the capacity of laboratory-based molecular SARS-CoV-2 testing, it has been suggested to combine several samples and jointly measure them in a sample pool. While saving cost and labour at first sight, pooling efficiency depends on the pool size and the presently experienced prevalence of positive samples. Here we address the question of the optimum pool size at a given prevalence. We demonstrate the relation between analytical effort and pool size and delineate the effects of the target prevalence on the optimum pool size. Finally, we derive a simple-to-use formula and table that allows laboratories performing sample pooling to assess the optimum pool size at a presently experienced target prevalence rate. |
| Databáze: | OpenAIRE |
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