Evaluation of specificity of indirect enzyme-linked immunosorbent assay for diagnosis of human Q fever
| Autor: | Daniel B. Fishbein, I J Uhaa, J C Williams, D M Waag, C C Rives, James G. Olson |
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| Rok vydání: | 1994 |
| Předmět: |
Microbiology (medical)
Rocky Mountain spotted fever Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Q fever Biology Sensitivity and Specificity Diagnosis Differential Antigen Seroepidemiologic Studies medicine Humans Rocky Mountain Spotted Fever Ehrlichiosis Antibody titer Reproducibility of Results Convalescence Typhus Endemic Flea-Borne bacterial infections and mycoses Coxiella burnetii biology.organism_classification medicine.disease Antibodies Bacterial Virology Rickettsiosis Evaluation Studies as Topic Acute Disease Immunology Ehrlichiosis (canine) biology.protein Antibody Q Fever Research Article |
| Zdroj: | Journal of Clinical Microbiology. 32:1560-1565 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.32.6.1560-1565.1994 |
| Popis: | Ninety-five acute- and convalescent-phase serum specimens from 48 patients suspected of having rickettsial or Legionella infections were assayed for antibodies to Coxiella burnetii, the causative agent of Q fever. To evaluate the specificity of the indirect enzyme-linked immunosorbent assay (ELISA) for human Q fever, we compared the ELISA results with those of the indirect immunofluorescence antibody (IFA) test. The ELISA data were analyzed by two different criteria for a positive test. The first criterion for positive results by ELISA was based upon diagnostic titers established in a study of 150 subjects who had no demonstrable cellular or humoral immune responses to C. burnetii phase I or phase II whole cells or phase I lipopolysaccharide. The second criterion was based upon diagnostic antibody titers in a study of 51 subjects who had been diagnosed as having clinical Q fever and had fourfold or greater rises in humoral immune responses to C. burnetii phase I and phase II whole-cell antigens. A comparison of the ELISA and IFA test results of the 95 serum specimens indicated excellent agreement between the tests (Kappa = 92.9%; P < 0.05). None of the 38 patients whose etiologies were confirmed serologically as Legionnaires' disease or rickettsial diseases other than Q fever were classified as positive for C. burnetii by the ELISA. Only one patient identified by the IFA test as having Q fever was not scored positive by the ELISA. These results suggest that the ELISA is useful for epidemiologic screening and as a diagnostic test for human Q fever. |
| Databáze: | OpenAIRE |
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