Effects of Electroacupuncture Combined with Stem Cell Transplantation on Anal Sphincter Injury-Induced Faecal Incontinence in a Rat Model
| Autor: | Jingen Lu, Xiutian Guo, Weiqi Jin, Xiaojia Li |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
medicine.medical_specialty Stromal cell Electroacupuncture medicine.medical_treatment Urology Anal Canal Stimulation Mesenchymal Stem Cell Transplantation Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Animals Humans Medicine Chemokine CCL7 business.industry Monocyte Therapeutic effect General Medicine Chemokine CXCL12 Rats Transplantation medicine.anatomical_structure Complementary and alternative medicine 030220 oncology & carcinogenesis Immunohistochemistry 030211 gastroenterology & hepatology Neurology (clinical) Stem cell business Fecal Incontinence |
| Zdroj: | Acupuncture in Medicine. 36:254-260 |
| ISSN: | 1759-9873 0964-5284 |
| DOI: | 10.1136/acupmed-2016-011262 |
| Popis: | Background Bone marrow mesenchymal stem cells (BMSCs) and acupuncture are known to mitigate tissue damage. This study aimed to investigate the therapeutic effects of combined electroacupuncture (EA) stimulation and BMSC injection in a rat model of anal sphincter injury-induced faecal incontinence (FI). Methods 60 Sprague-Dawley rats were randomly divided into five groups: sham-operated control, FI, FI+EA, FI+BMSC, and FI+BMSC+EA. The anorectal tissues were collected on days 1, 3, 7 and 14. Repair of the injured anal sphincter was compared using haematoxylin and eosin (HE) and immunocytochemiscal analyses with sarcomeric α actinin. The expression of stromal cell derived factor-1 (SDF-1) and monocyte chemoattractant protein-3 (MCP-3) was detected by quantitative reverse transcription PCR to evaluate the effects of EA on the homing of BMSCs. Results The therapeutic effect of combined EA+BMSCs on damaged tissue was the strongest among all the groups as indicated by HE and immunohistochemical staining. The expression of SDF-1 and MCP-3 was significantly increased by combined EA and BMSC treatment when compared with the other groups (P=0.01 to PConclusion The combination of EA and BMSC transplantation effectively repaired the impaired anal sphincters. The underlying mechanism might be associated with apparent promotive effects of EA on the homing of BMSCs. Our study provides a theoretical basis for the development of a non-surgical treatment method for FI secondary to muscle impairment. |
| Databáze: | OpenAIRE |
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