Determination of the hop-derived phytoestrogen, 8-prenylnaringenin, in beer by gas chromatography/mass spectrometry
| Autor: | Tekel' J, De Keukeleire D, Rong H, E. Daeseleire, Van Peteghem C |
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| Rok vydání: | 1999 |
| Předmět: |
Detection limit
Flavonoids Chromatography Extraction (chemistry) Ethyl acetate Beer Phytoestrogens General Chemistry Isoflavones Gas Chromatography-Mass Spectrometry Hop (networking) Matrix (chemical analysis) chemistry.chemical_compound chemistry Flavanones Humans Gas chromatography Estrogens Non-Steroidal Plant Preparations Diethyl ether General Agricultural and Biological Sciences 8-Prenylnaringenin |
| Zdroj: | Journal of agricultural and food chemistry. 47(12) |
| ISSN: | 0021-8561 |
| Popis: | A method was developed to determine 8-prenylnaringenin, a novel hop-derived phytoestrogen, in beer. Matrix purification involved solid-phase extraction on octadecyl silica followed by liquid/liquid extraction on a ChemElut 1010 column connected to a Florisil adsorption/desorption cartridge. 8-Prenylnaringenin was eluted from the tandem columns using a 1:1 mixture of diethyl ether and ethyl acetate and subsequently determined as tris(trimethylsilyl) ether by GC/MS-SIM. The recovery of 8-prenylnaringenin in beer samples was between 61.1 +/- 6.6 and 82.2 +/- 8.8% for levels of 37 and 92.5 microg L(-1), respectively, and the detection limit was approximately 5 microg L(-1). Although most beers do not contain 8-prenylnaringenin in detectable quantities, the highest concentration found was 19.8 microg L(-1). The concentration of 8-prenylnaringenin in beers and, possibly, its absence depend on the selection of particular hop varieties, the hopping rate, or the type of hop product used in brewing. The efficiency of transfer of 8-prenylnaringenin from hops to beer is between 10 and 20%. |
| Databáze: | OpenAIRE |
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