The control of transcriptional memory by stable mitotic bookmarking
| Autor: | Mounia Lagha, Melissa M. Harrison, George Hunt, Jeremy Dufourt, Antonio Trullo, Maelle Bellec, Cyril Favard, Jean-Christophe Andrau, Ovidiu Radulescu, Mattias Mannervik, Hélène Lenden-Hasse, Marissa M Gaskill, Marie Lamarque, Heloïse Faure-Gautron, Amal Zine El Aabidine |
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| Přispěvatelé: | Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
[SDV]Life Sciences [q-bio]
General Physics and Astronomy Mitosis General Biochemistry Genetics and Molecular Biology Histones 03 medical and health sciences Gene expression Animals Transcription factor 030304 developmental biology 0303 health sciences Multidisciplinary biology Bookmarking 030302 biochemistry & molecular biology Acetylation General Chemistry Chromatin Cell biology Histone Mitotic exit biology.protein Maternal to zygotic transition Drosophila Transcription Factors |
| Popis: | To maintain cellular identities during development, gene expression profiles must be faithfully propagated through cell generations. The reestablishment of gene expression patterns upon mitotic exit is thought to be mediated, in part, by mitotic bookmarking by transcription factors (TF). However, the mechanisms and functions of TF mitotic bookmarking during early embryogenesis remain poorly understood. In this study, taking advantage of the naturally synchronized mitoses of Drosophila early embryos, we provide evidence that the pioneer-like transcription factor GAF acts as stable mitotic bookmarker during zygotic genome activation. We report that GAF remains associated to a large fraction of its interphase targets including at cis-regulatory sequences of key developmental genes, with both active and repressive chromatin signatures. GAF mitotic targets are globally accessible during mitosis and are bookmarked via histone acetylation (H4K8ac). By monitoring the kinetics of transcriptional activation in living embryos, we provide evidence that GAF binding establishes competence for rapid activation upon mitotic exit. |
| Databáze: | OpenAIRE |
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