LED Lights Promote Growth and Flavonoid Accumulation of Anoectochilus roxburghii and Are Linked to the Enhanced Expression of Several Related Genes
| Autor: | Phan Thi Lan Anh, Ly Khanh Linh, Chu Hoang Ha, Nguyen Thi Thu, Do Thi Gam, Phan Hong Khoi, Nguyen Thi Hien, Pham Bich Ngoc, Tran Dang Khanh, Nguyen Khac Hung |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Chalcone synthase Anoectochillus roxburghii Flavonoid flavonol synthase Plant Science chalcone synthase 01 natural sciences Acclimatization Article 03 medical and health sciences Flavonol synthase flavonoid Dry matter Ecology Evolution Behavior and Systematics 030304 developmental biology chemistry.chemical_classification 0303 health sciences LED light Ecology biology Chemistry Botany biology.organism_classification Horticulture Flavonoid biosynthesis Seedling QK1-989 biology.protein 010606 plant biology & botany Explant culture |
| Zdroj: | Plants Volume 9 Issue 10 Plants, Vol 9, Iss 1344, p 1344 (2020) |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants9101344 |
| Popis: | Anoectochilus roxburghii is a wild edible species and has been traditionally used for a wide range of diseases in many countries. Our research aims to find the optimal light-emitting diode (LED) lighting conditions to improve the growth and development of A. roxburghii seedling at the acclimation stage. Two-month-old explants were cultured under the various lighting conditions including red (R), blue (B), BR (one blue: four red), BRW151 (one blue: five red: one white), BRW142 (one blue: four red: two white), and fluorescent lamp (FL). The results showed that the lighting conditions not only affect the growth and morphology of plants but also the accumulation of total flavonoids. Single wavelengths (B or R LED) inhibited the growth and secondary biosynthesis of A. roxburghii, while the BR LED showed an enhancement in both growth and biomass accumulation. A. roxburghii plants were grown under BR LED light has average plant height (7.18 cm), stem diameter (17.6mm), number of leaves (5.78 leaves/tree), leaf area (4.67 cm2), fresh weight (0.459 g/tree), dry matter percentages (11.69%), and total flavonoid (1.811 mg/g FW) is considered to be superior to FL lamps and other LEDs in the experiment. This indicates that both blue and red wavelengths are required for the normal growth of A. roxburghii. To learn more about how light affects flavonoid biosynthesis, we evaluated the expression of genes involved in this process (pal, chs, chi, and fls) and found that BR LED light enhances the expression level of chi and fls genes compared to fluorescent lamps (1.18 and 1.21 times, respectively), leading to an increase in the flavonoid content of plant. Therefore, applying BR LED during in vitro propagation of A. roxburghii could be a feasible way to improve the medicinal value of this plant. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|