Distribution of Adiponectin Receptors 1 and 2 in the Rat Olfactory Bulb and the Effect of Adiponectin Injection on Insulin Receptor Expression
| Autor: | Rosalinda Guevara-Guzmán, Octavio Fabián Mercado-Gómez, Alfredo Miranda-Martínez, Virginia Arriaga-Ávila |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty animal structures Article Subject Endocrinology Diabetes and Metabolism Olfaction lcsh:Diseases of the endocrine glands. Clinical endocrinology 03 medical and health sciences 0302 clinical medicine Endocrinology Western blot Internal medicine medicine Receptor Messenger RNA lcsh:RC648-665 Adiponectin biology medicine.diagnostic_test Endocrine and Autonomic Systems business.industry Olfactory bulb Insulin receptor 030104 developmental biology biology.protein business 030217 neurology & neurosurgery hormones hormone substitutes and hormone antagonists Research Article Hormone |
| Zdroj: | International Journal of Endocrinology, Vol 2017 (2017) International Journal of Endocrinology |
| ISSN: | 1687-8337 |
| DOI: | 10.1155/2017/4892609 |
| Popis: | Background. Adiponectin (APN) is an adipocyte-derived hormone that has peripheral beneficial effects. Although its receptors AdipoR1 and AdipoR2 are expressed in the brain, their function in neurons is poorly understood. The aims of this work were to describe the distribution of APN receptors in the olfactory bulb (OB) as well as the possible effects of APN injection on the insulin receptor (InsR) content and Akt kinase. Method. We performed the double immunofluorescence technique to describe the distribution of AdipoRs and the cellular type they were expressing. mRNA transcript and protein content were assessed by RT-PCR and Western blot, respectively. APN injection was performed to analyze its possible effect on the insulin pathway. Results. We found that AdipoRs were localized in all cell layers and in both neurons and astrocytes. We observed the presence of mRNA transcripts and immunoblot analysis confirmed the protein on the intact OB; APN injection in the OB resulted in a slight decrease of the total InsR and Akt phosphorylation and a reduction of phopho-InsR content. Conclusions. These data demonstrated that AdipoRs are expressed in OB regions, and APN injection could act as an insulin pathway modulator in the OB and thus possibly contribute to olfaction physiology. |
| Databáze: | OpenAIRE |
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