Approach to the isolation of antiproliferative genes
| Autor: | Mark J. Nuell, J.Keith McClung, David B. Danner, James R. Smith |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Aging
Microinjections Biology Biochemistry Endocrinology Genetics Animals Humans Vector (molecular biology) Genetic Testing RNA Messenger Cloning Molecular Molecular Biology Microinjection Gene Gene Library Cloning cDNA library RNA Rats Inbred Strains Cell Biology Molecular biology Fibronectins Rats Fibronectin Genes GenBank biology.protein Poly A Cell Division |
| Zdroj: | Experimental gerontology. 24(5-6) |
| ISSN: | 0531-5565 |
| Popis: | Poly(A) RNAs from normal rat liver and senescent human fibroblasts appear to have more antiproliferative activity than RNAs from regenerating rat liver and early passage human fibroblasts. We have screened two rat liver and one human liver library by differential hybridization and isolated four candidate cDNAs for this antiproliferative activity; one is fibronectin and three others do not match to any sequence in the mammalian portion of the GENBANK database. We are currently testing the antiproliferative nature of these cDNAs by microinjection of hybrid-selected RNA, and we describe an alternative strategy for cloning such genes based on construction of a cDNA library in an RNA expression vector. |
| Databáze: | OpenAIRE |
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