Microscopic and spectroscopic analysis of chitosan–DNA conjugates
| Autor: | Daniel Agudelo, H.A. Tajmir-Riahi, Laurent Kreplak |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Polymers and Plastics
Molecular Conformation 02 engineering and technology Microscopy Atomic Force 010402 general chemistry Photochemistry 01 natural sciences Chitosan chemistry.chemical_compound Materials Chemistry Organic chemistry chemistry.chemical_classification Aqueous solution Organic Chemistry technology industry and agriculture Chitosan binding Cationic polymerization DNA Polymer 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Particle 0210 nano-technology Conjugate |
| Zdroj: | Carbohydrate Polymers. 137:207-213 |
| ISSN: | 0144-8617 |
| Popis: | Conjugations of DNA with chitosans 15 kD (ch-15), 100 kD (ch-100) and 200 kD (ch-200) were investigated in aqueous solution at pH 5.5-6.5. Multiple spectroscopic methods and atomic force microscopy (AFM) were used to locate the chitosan binding sites and the effect of polymer conjugation on DNA compaction and particle formation. Structural analysis showed that chitosan-DNA conjugation is mainly via electrostatic interactions through polymer cationic charged NH2 and negatively charged backbone phosphate groups. As polymer size increases major DNA compaction and particle formation occurs. At high chitosan concentration major DNA structural changes observed indicating a partial B to A-DNA conformational transition. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect