Validation of factor VIII activity for monitoring standard and extended half-life products and correlation to thrombin generation assays
| Autor: | Karin Strandberg, Jan Astermark, Eva Norström, Myriam Martin, Vivian Lind, Cecilia Augustsson |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Haemophilia A
030204 cardiovascular system & hematology Hemophilia A Thrombin generation Correlation 03 medical and health sciences 0302 clinical medicine Thrombin medicine Humans Genetics (clinical) Blood coagulation test Chromatography Factor VIII Chromogenic business.industry Half-life Hematology General Medicine Factor VIII Activity medicine.disease Blood Coagulation Tests business 030215 immunology medicine.drug Half-Life |
| Zdroj: | Haemophilia : the official journal of the World Federation of HemophiliaREFERENCES. 27(3) |
| ISSN: | 1365-2516 |
| Popis: | Introduction Monitoring replacement therapy with standard and extended half-life (EHL) products is challenging, since one-stage assay (OSA) and chromogenic substrate assay (CSA) results may differ significantly. Recent recommendations include local validation of each new product with recovery within 20-30%, depending on activity level. Aim To validate factor VIII (FVIII) activity for monitoring products in clinical use on Atellica Coag and to correlate it with thrombin generation. Methods Plasma samples spiked with Advate® , Elocta® , Adynovi® , Nuwiq® , NovoEight® and Afstyla® (0.05, 0.20, 0.50 and 0.80 IU/ml) were analysed using Atellica Coag 360 with CSA-1 (Coatest SP) and CSA-2 (FVIII chromogenic), and OSA (Actin FS). Thrombin generation was performed using two thrombin generation assays (TGA-1 (Thrombinoscope) and TGA-2 (Technothrombin). Results All products at levels above 0.05 IU/ml, except Adynovi, showed acceptable recovery using CSA-1, whereas measurements using CSA-2 gave more results outside the target level. All products, except Afstyla, showed acceptable recovery using OSA. Correlation between CSA-1 and OSA was excellent (r2 =1.0) with biases of 6-32%, depending on FVIII product. A clear dose-response was seen for all thrombin generation parameters and products using both methods, except at low levels for lag time using TGA-1. With CSA-1 as an independent variable, the correlations to thrombin peak (measured with TGA-2) were good (r2 = .8-.9). Conclusion Our data revealed good correlation and acceptable bias between CSA and OSA using our sets of reagents, methods and analyser in spiked samples. Thrombin generation gave good correlation to CSA-1 factor activity and is a possible complement to factor activity assays. |
| Databáze: | OpenAIRE |
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