A cis-regulatory antisense RNA represses translation in Vibrio cholerae through extensive complementarity and proximity to the target locus
| Autor: | Maya Tsao-Wu, Ronak Mistry, Howard Y. Chang, Naomi Vather, Jane M. Liu, John Michael Replogle |
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| Rok vydání: | 2015 |
| Předmět: |
Untranslated region
Small RNA Monosaccharide Transport Proteins regulatory RNA Biology 03 medical and health sciences MtlA Bacterial Proteins Transcription (biology) Protein biosynthesis Coding region RNA Small Interfering Vibrio cholerae Base Pairing Molecular Biology Post-transcriptional regulation antisense RNA 030304 developmental biology Genetics 0303 health sciences 030306 microbiology mannitol Gene Expression Regulation Bacterial Cell Biology Research Papers Ribosomal binding site Antisense RNA Genetic Loci Protein Biosynthesis cis-acting RNA 5' Untranslated Regions Ribosomes post-transcriptional regulation Subcellular Fractions |
| Zdroj: | RNA Biology |
| DOI: | 10.6084/m9.figshare.1378750.v2 |
| Popis: | As with all facultative pathogens, Vibrio cholerae must optimize its cellular processes to adapt to different environments with varying carbon sources and to environmental stresses. More specifically, in order to metabolize mannitol, V. cholerae must regulate the synthesis of MtlA, a mannitol transporter protein produced exclusively in the presence of mannitol. We previously showed that a cis-acting small RNA (sRNA) expressed by V. cholerae, MtlS, appears to post-transcriptionally downregulate the expression of mtlA and is produced in the absence of mannitol. We hypothesized that since it is complementary to the 5′ untranslated region (UTR) of mtlA mRNA, MtlS may affect synthesis of MtlA by forming an mtlA-MtlS complex that blocks translation of the mRNA through occlusion of its ribosome binding site. To test this hypothesis, we used in vitro translation assays in order to examine the role MtlS plays in mtlA regulation and found that MtlS is sufficient to suppress translation of transcripts harboring the 5′ UTR of mtlA. However, in a cellular context, the 5′ UTR of mtlA is not sufficient for targeted repression by endogenous MtlS; additional segments from the coding region of mtlA play a role in the ability of the sRNA to regulate translation of mtlA mRNA. Additionally, proximity of transcription sites between the sRNA and mRNA significantly affects the efficacy of MtlS. |
| Databáze: | OpenAIRE |
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