2-Deoxy-glucose Enhances the Effect of Cisplatin and Pemetrexed in Reducing Malignant Pleural Mesothelioma Cell Proliferation But Not Spheroid Growth
| Autor: | Eleftherios Papazoglou, Rajesh Jagirdar, Chrissi Hatzoglou, Olympia Kouliou, Eleanna Pitaraki, Konstantinos I. Gourgoulianis, Irini Gerogianni, Sotirios G. Zarogiannis, Stefanos Giannopoulos, Lydia Giannakou |
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| Rok vydání: | 2019 |
| Předmět: |
Mesothelioma
Cancer Research Lung Neoplasms Cell Survival Pleural Neoplasms medicine.medical_treatment Antineoplastic Agents Pemetrexed Deoxyglucose 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Spheroids Cellular medicine Humans Neoplasm Viability assay Cell Proliferation Cisplatin Chemotherapy Cell growth Chemistry Mesothelioma Malignant Spheroid General Medicine medicine.disease Oncology Cell culture 030220 oncology & carcinogenesis Cancer research medicine.drug |
| Zdroj: | Anticancer Research. 39:3809-3814 |
| ISSN: | 1791-7530 0250-7005 |
| DOI: | 10.21873/anticanres.13530 |
| Popis: | BACKGROUND/AIM Malignant pleural mesothelioma (MPM) is a therapy-resistant neoplasm of the pleura. Standard chemotherapy consists of a combination of cisplatin (CPDD) and pemetrexed (PEM). The aim of this study was to assess whether inhibition of aerobic glycolysis by 2-deoxy-glucose (2DG) would enhance the effects of standard chemotherapy. MATERIALS AND METHODS MeT-5A, M14K, MSTO and ZL34 cell lines were used. Cell viability with 2DG and cell proliferation and spheroid formation with CPDD+PEM alone and with 2-DG were tested. RESULTS Viability with 2-DG was dose-dependent. Cell proliferation with CPDD+PEM on 2D surface was reduced in all cell types, 2-DG inclusion demonstrated a synergistic effect in MSTO and ZL34 cells. Spheroid growth in 3D with CPDD+PEM or CPDD+PEM+2-DG lowered spheroid growth in all cell types. CONCLUSION 2-DG synergizes with CPDD+PEM in lowering MPM cell proliferation in 2D to |
| Databáze: | OpenAIRE |
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