Comparative study of hyperpure chlorine dioxide with two other irrigants regarding the viability of periodontal ligament stem cells
Autor: | László Kőhidai, Zsolt Lohinai, Anna Herczegh, Gábor Varga, Júlia Láng, Orsolya Láng, Krisztina Nagy, Katalin Perczel-Kovách |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Periodontal ligament stem cells Periodontal Ligament Disinfectant 030106 microbiology PDLSC Pharmacology Stem cell marker Chlorine dioxide 03 medical and health sciences 0302 clinical medicine medicine CD90 Viability assay General Dentistry Dental structure Toxicity Chemistry Stem Cells Chlorhexidine Mesenchymal stem cell Cell Differentiation Oxides Hydrogen Peroxide 030206 dentistry Viability Dental stem cells Original Article Chlorine Compounds medicine.drug |
Zdroj: | Clinical Oral Investigations |
ISSN: | 1436-3771 1432-6981 |
Popis: | Objectives Periodontal ligament stem cells (PDLSCs) have an underlined significance as their high proliferative capacity and multipotent differentiation provide an important therapeutic potential. The integrity of these cells is frequently disturbed by the routinely used irrigative compounds applied as periodontal or endodontic disinfectants (e.g., hydrogen peroxide (H2O2) and chlorhexidine (CHX)). Our objectives were (i) to monitor the cytotoxic effect of a novel dental irrigative compound, chlorine dioxide (ClO2), compared to two traditional agents (H2O2, CHX) on PDLSCs and (ii) to test whether the aging factor of PDLSC cultures determines cellular responsiveness to the chemicals tested. Methods Impedimetry (concentration-response study), WST-1 assays (WST = water soluble tetrazolium salt), and morphology analysis were performed to measure changes in cell viability induced by the 3 disinfectants; immunocytochemistry of stem cell markers (STRO-1, CD90, and CD105) measured the induced mesenchymal characteristics. Results Cell viability experiments demonstrated that the application of ClO2 does not lead to a significant decrease in viability of PLDSCs in concentrations used to kill microbes. On the contrary, traditional irrigants, H2O2, and CHX are highly toxic on PDLSCs. Aging of PLDSC cultures (passages 3 vs. 7) has characteristic effects on their responsiveness to these agents as the increased expression of mesenchymal stem cell markers turns to decreased. Conclusions and clinical relevance While the active ingredients of mouthwash (H2O2, CHX) applied in endodontic or periodontitis management have a serious toxic effect on PDLSCs, the novel hyperpure ClO2 is less toxic providing an environment favoring dental structure regenerations during disinfectant interventions. |
Databáze: | OpenAIRE |
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