Insertional Inactivation of Virulence Operon in Population of Persistent Bordetella pertussis Bacteria
| Autor: | I. G. Kondzariya, L. N. Sinyashina, E. G. Semin, Z. Ya. Mikvabia, A. Z. Matua, A. A. Amichba, Z. V. Shevtsova, D. T. Kubrava, A. Yu. Medkova, G. I. Karataev |
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| Předmět: |
0301 basic medicine
Bordetella pertussis Bacilli education.field_of_study biology Operon Population Virulence biology.organism_classification medicine.disease Virology Microbiology 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Genetics medicine education Whooping cough Bacteria Respiratory tract |
| Zdroj: | Scopus-Elsevier |
| Popis: | Avirulent B. pertussis bacteria containing IS elements in the bvgAS operon were detected during the study of whooping cough patients and bacilli carriers. The present work is devoted to the study of the accumulation dynamics and the mechanisms of generation of persistent forms of the B. pertussis bacteria in lower monkeys as the most adequate model for extrapolation of the experiment results to humans. By means of the real-time PCR method, it was established that the B. pertussis bacteria lived more than three months in the upper respiratory tract after a single intranasal monkey infection; the period was reduced to 14–28 days during repeated infection. An increase in the portion of B. pertussis Bvg mutants in the population to tens of percent from the total number of registered bacteria was registered. The experimental confirmation of the development and accumulation of avirulent B. pertussis Bvg mutants during the development of the infectious process was obtained. Further study of the composition of the B. pertussis persistent bacteria population at different stages of the disease will make it possible to formulate new approaches to the whooping cough diagnostics and prevention and creation of fundamentally new drugs. |
| Databáze: | OpenAIRE |
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