MicroRNA‑1915‑3p prevents the apoptosis of lung cancer cells by downregulating DRG2 and PBX2
| Autor: | Ling Zhang, Lianning Duan, Chengshan Xu, Hengheng Li, Tianjun Jia, Chengrong Lu |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Cancer Research Lung Neoplasms Molecular Sequence Data Down-Regulation Apoptosis Biology Biochemistry 03 medical and health sciences 0302 clinical medicine GTP-Binding Proteins Cell Line Tumor Proto-Oncogene Proteins microRNA Genetics medicine Humans Lung cancer Molecular Biology Regulation of gene expression Homeodomain Proteins Oncogene Base Sequence Cancer Suicide gene Cell cycle medicine.disease Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cancer research Molecular Medicine |
| Zdroj: | Molecular medicine reports. 13(1) |
| ISSN: | 1791-3004 |
| Popis: | Micro (mi)RNAs are short non‑coding RNA molecules, which post‑transcriptionally regulate gene expression and exert key roles in cell growth, differentiation and apoptosis. In the present study, the mechanism and the function of miR‑1915‑3p in the apoptotic regulation of lung cancer cell lines (NCI‑H441 and NCI‑H1650) were investigated. The expression analysis confirmed that the expression of miR‑1915‑3p was markedly decreased in the apoptotic cells. The overexpression of miR‑1915‑3p in the lung cancer cells prevented apoptosis induced by etoposide. Developmentally regulated GTP‑binding protein 2 (DRG2) and pre‑B cell leukemia homeobox 2 (PBX2) were identified as downstream targets of miR‑1915‑3p, which was shown to bind directly to the 3'‑untranslated region of DRG2 and PBX2, subsequently lowering their mRNA and protein expression levels. Co‑expression of miR‑1915‑3p and DRG2/PBX2 in the NCI‑H441 and NCI‑H1650 cells partly circumvented the effect of miR‑1915‑3p on apoptosis. The results in the present study revealed that miR‑1915‑3p functions as a silencer of apoptosis, which regulates lung cancer apoptosis via targeting DRG2/PBX2, and consequently this miRNA may be a putative therapeutic target in lung cancer. |
| Databáze: | OpenAIRE |
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