Fluorescence Enhancement Effect by Metal Nanoparticles-immobilized Microplate

Autor:	Shuyi Sun, Hiroshi Shiigi, Takamasa Kinoshita, Kyohei Matsui, Dung Nguyen, So Tanabe, Yojiro Yamamoto
Rok vydání:	2020
Předmět:	Indirect elisa Silver Chemistry Nanoparticle Metal Nanoparticles Enzyme-Linked Immunosorbent Assay Fluorescence Silver nanoparticle Analytical Chemistry Highly sensitive Metal visual_art visual_art.visual_art_medium Gold Metal nanoparticles Palladium Nuclear chemistry
Zdroj:	Analytical sciences : the international journal of the Japan Society for Analytical Chemistry. 36(7)
ISSN:	1348-2246
Popis:	In this reported work, we achieved high-throughput, highly sensitive fluorescent analysis using an enzyme-linked immunosorbent assay (ELISA) that employed a metallic nanoparticle (NP)-immobilized 96-well plate. The immobilization of metallic NPs on a 96-well plate effectively amplified fluorescent signals of the assay. The silver (Ag) NP-immobilized plate showed the best fluorescent enhancement effect of all plates immobilized by metal NPs. Our results demonstrate the potential of applying Ag NPs to enhance the efficiency of direct and indirect ELISA by the labeling of targets.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::162c6c52fd7f3c5fb561c3d134185365 https://pubmed.ncbi.nlm.nih.gov/32595192 Zobrazit plný text záznamu Full text from SpringerLink