Pandemic, Epidemic, Endemic: B Cell Repertoire Analysis Reveals Unique Anti-Viral Responses to SARS-CoV-2, Ebola and Respiratory Syncytial Virus
| Autor: | Alexander Stewart, Emma Sinclair, Joseph Chi-Fung Ng, Joselli Silva O’Hare, Audrey Page, Ilaria Serangeli, Christian Margreitter, Federica Orsenigo, Katherine Longman, Cecile Frampas, Catia Costa, Holly-May Lewis, Nora Kasar, Bryan Wu, David Kipling, Peter JM Openshaw, Christopher Chiu, J Kenneth Baillie, Janet T. Scott, Malcolm G. Semple, Melanie J. Bailey, Franca Fraternali, Deborah K. Dunn-Walters |
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| Přispěvatelé: | Stewart, A, Sinclair, E, Ng, J, O'Hare, J, Page, A, Serangeli, I, Margreitter, C, Orsenigo, F, Longman, K, Frampas, C, Costa, C, Lewis, H, Kasar, N, Wu, B, Kipling, D, Openshaw, P, Chiu, C, Baillie, J, Scott, J, Semple, M, Bailey, M, Fraternali, F, Dunn-Walters, D, Medical Research Council (MRC), National Institute for Health Research, UKRI MRC COVID-19 Rapid Response Call |
| Rok vydání: | 2022 |
| Předmět: |
MECHANISM
ANTIBODY-RESPONSES HYPERMUTATION class-switch recombination (CSR) ebola antibody sequencing bioinformatics Immunology Antibodies Viral CYTIDINE DEAMINASE AID RSV (respiratory syncytial virus) 1108 Medical Microbiology INFECTION Humans Immunology and Allergy Pandemics B cell Science & Technology IGM SARS-CoV-2 MEMORY repertoire COVID-19 Hemorrhagic Fever Ebola Ebolavirus immunity 1107 Immunology Respiratory Syncytial Virus Human Life Sciences & Biomedicine CLASS SWITCH RECOMBINATION |
| Zdroj: | Stewart, A, Sinclair, E, Ng, J C-F, O'Hare, J S, Page, A, Serangeli, I, Margreitter, C, Orsenigo, F, Longman, K, Frampas, C, Costa, C, Lewis, H-M, Kasar, N, Wu, B, Kipling, D, Openshaw, P J, Chiu, C, Baillie, J K, Scott, J T, Semple, M G, Bailey, M J, Fraternali, F & Dunn-Walters, D K 2022, ' Pandemic, Epidemic, Endemic : B Cell Repertoire Analysis Reveals Unique Anti-Viral Responses to SARS-CoV-2, Ebola and Respiratory Syncytial Virus ', Frontiers in Immunology, vol. 13, 807104 . https://doi.org/10.3389/fimmu.2022.807104 |
| ISSN: | 1664-3224 |
| DOI: | 10.3389/fimmu.2022.807104 |
| Popis: | Immunoglobulin gene heterogeneity reflects the diversity and focus of the humoral immune response towards different infections, enabling inference of B cell development processes. Detailed compositional and lineage analysis of long read IGH repertoire sequencing, combining examples of pandemic, epidemic and endemic viral infections with control and vaccination samples, demonstrates general responses including increased use of IGHV4-39 in both Zaire Ebolavirus (EBOV) and COVID-19 patient cohorts. We also show unique characteristics absent in Respiratory Syncytial Virus or yellow fever vaccine samples: EBOV survivors show unprecedented high levels of class switching events while COVID-19 repertoires from acute disease appear underdeveloped. Despite the high levels of clonal expansion in COVID-19 IgG1 repertoires there is a striking lack of evidence of germinal centre mutation and selection. Given the differences in COVID-19 morbidity and mortality with age, it is also pertinent that we find significant differences in repertoire characteristics between young and old patients. Our data supports the hypothesis that a primary viral challenge can result in a strong but immature humoral response where failures in selection of the repertoire risk off-target effects. |
| Databáze: | OpenAIRE |
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