Uniformly 14C-labelled plant cell walls: Production, analysis and behaviour in rat gastrointestinal tract
| Autor: | S. C. Fry, Martin Eastwood, D. F. Gray |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Dietary Fiber
Male Starch Medicine (miscellaneous) Biology Polysaccharide Cell wall chemistry.chemical_compound Cell Wall Polysaccharides Animals Lignin Carbon Radioisotopes Food science Rats Wistar Cellulose chemistry.chemical_classification Nutrition and Dietetics Carbohydrate Rats Xyloglucan chemistry Biochemistry Fermentation Plants Edible Digestion Digestive System |
| Zdroj: | British Journal of Nutrition. 69:177-188 |
| ISSN: | 1475-2662 0007-1145 |
| DOI: | 10.1079/bjn19930020 |
| Popis: | Uniformly 14C-labelled primary cell walls (14C-PCW) were purified from suspension-cultured cells of spinach (Spinacia oleracea L.) grown in a medium containing D-[U-14C]glucose. The approximate polymer composition of the 14C-PCW preparation (% total 14C) was homogalacturonan 30,rhamnogalacturonan 23, xyloglucan 10, other hemicelluloses 3, cellulose 21, lignin 0,14C-labelled protein < 3 and [14C]starch < 2. The degree of methyl esterification of the pectic polysaccharides was about 25%. The 14C-PCW contained about 4% O-acetyl and 3% non-volatile ester-linked residues. When tracer levels of these 14C-PCW were fed to rats, only about 18% of the 14C appeared in the faeces;negligible levels of 14C (0.07%) remained in the gut contents 4 d after feeding. Some 14C was present in the carcass. The results show that U-14C-labelled primary cell walls can be purified and radiochemically analysed by the methods developed here, and that primary cell walls are extensively fermented by the gut microflora of the rat. |
| Databáze: | OpenAIRE |
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