Halobaculum roseum sp. nov., isolated from underground salt deposits
| Autor: | An-Na Yang, Shaoxing Chen, Hong-Can Liu, Yao Xu, Li-Xia Ke |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
China Halobaculum gomorrense 030106 microbiology Sodium Chloride Microbiology Mining 03 medical and health sciences chemistry.chemical_compound RNA Ribosomal 16S Phospholipids Phylogeny Ecology Evolution Behavior and Systematics Phosphatidylglycerol Base Composition Halobacteriaceae biology Strain (chemistry) Nucleic Acid Hybridization Phosphatidylglycerols Sequence Analysis DNA General Medicine rpoB 16S ribosomal RNA biology.organism_classification Halophile DNA Archaeal chemistry Haloarchaea Glycolipids Halobaculum |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 67:818-823 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.001663 |
| Popis: | Two extremely halophilic archaea, strains D90T and D93, were isolated from underground salt deposits of Yunnan salt mine, China. Both strains were pleomorphic or short rods, non-motile, Gram-negative and required 1.7 M NaCl for growth, with optimum at 3.4 M. Mg2+ was not required for growth. Multiple copies of the 16S rRNA gene were obtained for both strains. Sequence similarity analysis of 16S rRNA genes revealed that strains D90T and D93 were closely related to Halobaculum magnesiiphilum MGY-184T and Halobaculum gomorrense DSM 9297T with the sequence similarity between 96.2–98.1 %. The sequence similarity of the rpoB′ gene between strain D90T and Halobaculum gomorrense JCM 9908T was 94.1 %. The DNA G+C contents of strains D90T and D93 were 65.9 and 67.6 mol%, respectively. The major polar lipids of both strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and glycolipid. The DNA–DNA relatedness value between strains D90T and D93 was 90.1±0.5 %, while that between strain D90T and Halobaculum gomorrense JCM 9908T was 30.0±0.7 %. The comparison of physiological and biochemical characteristics, including the requirements of NaCl, Mg2+, pH, etc., differentiated strains D90T and D93 from Halobaculum magnesiiphilum MGY-184T and Halobaculum gomorrense JCM 9908T. Therefore, strains D90T and D93 represent a novel species of the genus Halobaculum , for which the name Halobaculum roseum sp. nov. is proposed. The type strain is D90T (=CGMCC 1.15501T=JCM 31273T). |
| Databáze: | OpenAIRE |
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