Evaluation of antioxidant status and oxidative stress in sheep naturally infected with Babesia ovis
| Autor: | Bahram Dalir-Naghadeh, Bijan Esmaeilnejad, Siamak Asri-Rezaei, Mosa Tavassoli |
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| Rok vydání: | 2011 |
| Předmět: |
Babesia
Sheep Diseases Parasitemia medicine.disease_cause Antioxidants Superoxide dismutase Andrology chemistry.chemical_compound Babesiosis medicine Animals Ovis chemistry.chemical_classification Sheep General Veterinary biology Glutathione peroxidase Anemia General Medicine biology.organism_classification Malondialdehyde medicine.disease Red blood cell Oxidative Stress medicine.anatomical_structure chemistry Immunology biology.protein Parasitology Hemoglobin Lipid Peroxidation Oxidative stress |
| Zdroj: | Veterinary parasitology. 185(2-4) |
| ISSN: | 1873-2550 |
| Popis: | The present study aimed to assess antioxidant status and oxidative stress in sheep naturally infected with Babesia ovis. Red blood cell (RBC) count, hemoglobin (Hb) concentration, packed cell volume (PCV), piroplasm parasitemia percentage, malondialdehyde (MDA) concentration, erythrocyte superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activities and total antioxidant capacity (TAC) were determined in 52 sheep naturally infected with B. ovis as well as same number of healthy sheep in West-Azerbaijan province, Iran. Microscopic examination of Giemsa-stained peripheral blood smears revealed B. ovis infection. The parasitological diagnosis was confirmed using polymerase chain reaction (PCR) analysis by amplifying a partial small subunit ribosomal RNA (ssu rRNA) gene sequence of B. ovis of 52 diseased sheep, 18 (34.61%), 11 (21.15%), 16 (30.76%) and 7 (13.48%) had1%, 1-2%, 2-3% and3% parasitemia, respectively. Compared to controls, the activities of erythrocyte GSH-Px, SOD, TAC and CAT showed a significant decrease, whereas the concentration of MDA in erythrocytes of infected sheep increased significantly. Parasitemia rate was positively correlated with MDA and negatively correlated with PCV, SOD, CAT, GSH-Px and TAC. Also, MDA was negatively correlated with PCV, SOD, catalase, GSH-Px and TAC. The study demonstrated that B. ovis plays an important role in the occurrence of oxidative damage to RBCs and anemia in ovine babesiosis. |
| Databáze: | OpenAIRE |
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