Bicarbonate Evokes Reciprocal Changes in Intracellular Cyclic di-GMP and Cyclic AMP Levels in Pseudomonas aeruginosa
| Autor: | Roland Tengölics, Balázs Stercz, Mária A. Deli, Pongsiri Jaikumpun, Orsolya Dobay, Zsolt Lohinai, Gergő Tóth, Ákos Zsembery, Kasidid Ruksakiet, Martin C. Steward, Peter Horvath, Ilona Gróf |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cyclic di-GMP P. aeruginosa QH301-705.5 Bicarbonate 030106 microbiology bicarbonate Biology medicine.disease_cause Article General Biochemistry Genetics and Molecular Biology biofilm Microbiology cystic fibrosis 03 medical and health sciences chemistry.chemical_compound cAMP medicine Extracellular Biology (General) Incubation General Immunology and Microbiology Pseudomonas aeruginosa pH Biofilm c-di-GMP chronic infection 030104 developmental biology chemistry Second messenger system General Agricultural and Biological Sciences Intracellular |
| Zdroj: | Biology Volume 10 Issue 6 Biology, Vol 10, Iss 519, p 519 (2021) |
| ISSN: | 2079-7737 |
| DOI: | 10.3390/biology10060519 |
| Popis: | The formation of Pseudomonas aeruginosa biofilms in cystic fibrosis (CF) is one of the most common causes of morbidity and mortality in CF patients. Cyclic di-GMP and cyclic AMP are second messengers regulating the bacterial lifestyle transition in response to environmental signals. We aimed to investigate the effects of extracellular pH and bicarbonate on intracellular c-di-GMP and cAMP levels, and on biofilm formation. P. aeruginosa was inoculated in a brain–heart infusion medium supplemented with 25 and 50 mM NaCl in ambient air (pH adjusted to 7.4 and 7.7 respectively), or with 25 and 50 mM NaHCO3 in 5% CO2 (pH 7.4 and 7.7). After 16 h incubation, c-di-GMP and cAMP were extracted and their concentrations determined. Biofilm formation was investigated using an xCelligence real-time cell analyzer and by crystal violet assay. Our results show that HCO3− exposure decreased c-di-GMP and increased cAMP levels in a dose-dependent manner. Biofilm formation was also reduced after 48 h exposure to HCO3−. The reciprocal changes in second messenger concentrations were not influenced by changes in medium pH or osmolality. These findings indicate that HCO3− per se modulates the levels of c-di-GMP and cAMP, thereby inhibiting biofilm formation and promoting the planktonic lifestyle of the bacteria. |
| Databáze: | OpenAIRE |
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