Differences in electrophoretic patterns of non-histone proteins from large and small nuclei of rat brain
| Autor: | H. P. von Hahn, H. R. Olpe, C. G. Honegger |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Brain Chemistry
Cell Nucleus Cerebral Cortex General Neuroscience Nerve Tissue Proteins Modified method Sucrose gradient Biology Electrophoresis Disc Rat brain Rats Electrophoresis Histone medicine.anatomical_structure Liver Biochemistry Solubilization Cortex (anatomy) biology.protein medicine Animals Neurology (clinical) Nuclear protein Molecular Biology Developmental Biology |
| Zdroj: | Brain Research. 58:453-464 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/0006-8993(73)90014-0 |
| Popis: | Non-histone proteins from total and fractionated nuclei of brain and of total nuclei of liver have been extracted with a modified method of Gronow and Griffiths 8 and separated by disc electrophoresis. Non-histone proteins extracted from total brain nuclei could be separated into 14 main bands based on molecular weight. With non-histone proteins extracted from total liver nuclei, only 12 bands were obtained. The comparison of both electrophoretic patterns indicated a limited tissue specificity for these proteins. Total brain and cortex nuclei were fractionated into large and small nuclei on a discontinuous sucrose gradient. The non-histone protein profiles of these two fractions for both total brain and cortex showed qualitative differences. In large nuclei non-histone proteins could be separated into 13 distinct bands, whereas only 10 bands were obtained for the small nuclei. In both large and small nuclei one specific band was found. Total brain and cortex gave similar protein profiles for the corresponding nuclear fractions. When total nuclei were prewashed with NaCl solution, 3.5% of total nuclear proteins could be extracted with 0.15 M NaCl and 13% with 0.35 M NaCl. One-half of the Gronow and Griffiths 8 soluble non-histone proteins were solubilized during the 0.35 M NaCl treatment. |
| Databáze: | OpenAIRE |
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