The uremic solute indoxyl sulfate acts as an antioxidant against superoxide anion radicals under normal-physiological conditions
| Autor: | Daisuke Kadowaki, Hiroshi Watanabe, Yasunori Iwao, Keizo Sato, Yu Ishima, Toru Maruyama, Yuka Tasaki, Yohei Miyamoto, Masaki Otagiri |
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| Rok vydání: | 2010 |
| Předmět: |
Antioxidant
Chemiluminescence Scavenging activity Neutrophils medicine.medical_treatment Radical Biophysics Allopurinol Biochemistry Antioxidants Adduct Indoxyl sulfate chemistry.chemical_compound Structural Biology Superoxides Genetics medicine Humans Pyrroles Xanthine oxidase Molecular Biology chemistry.chemical_classification Reactive oxygen species Superoxide Neutrophil Electron Spin Resonance Spectroscopy Cell Biology Xanthine chemistry Electron paramagnetic resonance Indican medicine.drug Superoxide anion radical |
| Zdroj: | FEBS letters. 584(13) |
| ISSN: | 1873-3468 |
| Popis: | The effect of the uremic solute indoxyl sulfate (IS) on scavenging superoxide anion radicals (O 2 ) generated from both the xanthine/xanthine oxidase (X/XO) system and activated neutrophils was investigated by electron paramagnetic resonance spectroscopy, combined with 2-ethoxycarbonyl2-methyl-3,4-dihydro-2H-pyrrole-1-oxide (EMPO). The findings show that the presence of normalphysiological serum concentrations of IS (0.1–10 lM) resulted in decreased formation of EMPOsuperoxide adduct without affecting XO activity. Furthermore, IS showed scavenging activity against cell-derived O 2 generated from activated neutrophils. In addition, IS also eliminated hydroxyl radicals. These findings suggest that IS acts as a novel endogenous antioxidant under normal-physiological conditions. 2010 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies. |
| Databáze: | OpenAIRE |
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