Solid-phase oligonucleotide synthesis: a renaissance

Autor:	R.C. Sheppard, M.J. Gait
Rok vydání:	1978
Předmět:	Chromatography Biochemistry Oligonucleotide Phase (matter) Genetics The Renaissance Articles Oligonucleotide synthesis Biology High pressure liquid chromatography procedure
Zdroj:	Nucleic Acids Research. 5:s79-s84
ISSN:	1362-4962 0305-1048
DOI:	10.1093/nar/1.suppl_1.s79
Popis:	A practical solid-phase method is described using a polar polyamide support for the synthesis of medium-length oligodeoxyribonucleotides. Chain assembly can be facilitated with the aid of a modified solid-phase peptide synthesiser. Hplc on both analytical and preparative scale allows rapid monitoring of syntheses as well as efficient final purification of the desired oligonucleotide.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::160ba7d191d51b848dd13a95d33216f6 https://doi.org/10.1093/nar/1.suppl_1.s79 Zobrazit plný text záznamu