Nanocrystalline diamond-based impedance sensors for real-time monitoring of adipose tissue-derived stem cells
Autor: | Tibor Ižák, Elena Filova, Lucie Bacakova, Alexander Kromka, Ondrej Szabó, Vít Jirásek, Václav Procházka, Jana Štěpanovská, Roman Matějka |
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Rok vydání: | 2019 |
Předmět: |
Time Factors
Materials science Phase (waves) 02 engineering and technology engineering.material 01 natural sciences Colloid and Surface Chemistry Live cell imaging 0103 physical sciences Electric Impedance Humans Physical and Theoretical Chemistry Thin film Electrical impedance Cells Cultured 010304 chemical physics business.industry Stem Cells Diamond Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology Dielectric spectroscopy Adipose Tissue Electrode engineering Nanoparticles Optoelectronics 0210 nano-technology business Biosensor Biotechnology |
Zdroj: | Colloids and Surfaces B: Biointerfaces. 177:130-136 |
ISSN: | 0927-7765 |
Popis: | Cell-based impedance spectroscopy is a promising label-free method for electrical monitoring of cell activity. Here we present a diamond-based impedance sensor with built-in gold interdigitated electrodes (IDT) as a promising platform for simultaneous electrical and optical monitoring of adipose tissue-derived stem cells (ASCs). The impedance spectra were collected in a wide frequency range (from 100 Hz to 50 kHz) for 90 h of cell cultivation in chambers designed for static cultivation. Absolute impedance spectra were analyzed in terms of measured frequencies and cell properties monitored by a high-resolution digital camera. The control commercially-available impedance system, based on gold electrodes exposed to the cultivation media, and also our specially developed sensor with gold electrodes built into a diamond thin film detected three phases of cell growth, namely the phase of cell attachment and spreading, the phase of cell proliferation, and the stationary phase without significant changes in cell number. These results were confirmed by simultaneous live cell imaging. The design of the sensing electrode is discussed, pointing out its enhanced sensitivity for a certain case. The diamond-based sensor appeared to be more sensitive for detecting the cell-substrate interaction in the first phase of cell growth, while the control system was more sensitive in the second phase of cell growth. |
Databáze: | OpenAIRE |
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