Citrus aurantium L. Dry Extracts Ameliorate Adipocyte Differentiation of 3T3-L1 Cells Exposed to TNFα by Down-Regulating miR-155 Expression
| Autor: | Giuseppe Cacace, Francesco Beguinot, Claudia Miele, Gregory Alexander Raciti, Sonia de Simone, Michele Campitelli, Pietro Campiglia, Alessia Leone, Pietro Formisano, Immacolata Prevenzano, Antonella Desiderio, Cecilia Nigro |
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| Přispěvatelé: | Campitelli, Michele, Desiderio, Antonella, Cacace, Giuseppe, Nigro, Cecilia, Prevenzano, Immacolata, Leone, Alessia, de Simone, Sonia, Campiglia, Pietro, Formisano, Pietro, Raciti, Gregory A, Beguinot, Francesco, Miele, Claudia |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
miR-155 030209 endocrinology & metabolism lcsh:TX341-641 CREB Citrus aurantium L. dry extract Article adipogenesis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Citrus aurantium L. dry extracts tumor necrosis factor-alpha Adipocyte microRNA Gene expression Cyclic adenosine monophosphate adipogenesi Nutrition and Dietetics biology Chemistry food and beverages Molecular biology 030104 developmental biology Adipogenesis biology.protein Tumor necrosis factor alpha lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Nutrients Volume 12 Issue 6 Nutrients 12 (2020). doi:10.3390/nu12061587 info:cnr-pdr/source/autori:Campitelli, Michele; Desiderio, Antonella; Cacace, Giuseppe; Nigro, Cecilia; Prevenzano, Immacolata; Leone, Alessia; de Simone, Sonia; Campiglia, Pietro; Formisano, Pietro; Raciti, Gregory A.; Beguinot, Francesco; Miele, Claudia/titolo:Citrus aurantium L. Dry extracts ameliorate adipocyte differentiation of 3T3-l1 cells exposed to tnf? by down-regulating MiR-155 expression/doi:10.3390%2Fnu12061587/rivista:Nutrients/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:12 Nutrients, Vol 12, Iss 1587, p 1587 (2020) |
| DOI: | 10.3390/nu12061587 |
| Popis: | Citrus aurantium L. dry extracts (CAde) improve adipogenesis in vitro. These effects are dependent from an early modulation of CCAAT/enhancer-binding protein beta (C/Ebp&beta ) expression and cyclic Adenosine Monophosphate (cAMP) response element-binding protein (CREB) activation. C/Ebp&beta and Creb are also targets of miR-155. This study investigated whether CAde regulates miR-155 expression in the early stages of adipogenesis and whether it ameliorates adipocyte differentiation of cells exposed to tumor necrosis factor-alpha (TNF&alpha ). Adipogenic stimuli (AS) were performed in 3T3-L1 pre-adipocytes treated with CAde, TNF&alpha or both. Gene and miRNA expression were determined by quantitative real-time PCR. Adipogenesis was evaluated by Oil-Red O staining. CAde treatment enhanced AS effects during the early adipogenesis phases by further down-regulating miR-155 expression and increasing both C/Ebp&beta and Creb mRNA and protein levels. At variance, TNF&alpha inhibited 3T3-L1 adipogenesis and abolished AS effects on miR-155, C/Ebp&beta and Creb expression. However, in cells exposed to TNF&alpha CAde improved adipocyte differentiation and restored the AS effects on miRNA and gene expression at early time points. In conclusion, this study identified miR-155 down-regulation as part of the mechanism through which CAde enhances adipogenesis of pre-adipocytes in vitro. Furthermore, it provides evidence of CAde efficacy against TNF&alpha negative effects on adipogenesis. |
| Databáze: | OpenAIRE |
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