Reduced expression of neurofibromin in human meningiomas
| Autor: | Jyoti A. Harwalkar, D. W. Stacey, D. J. Stein, Joung H. Lee, Vidyodhaya Sundaram, Margaret Roudebush, M. Golubic |
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| Rok vydání: | 1997 |
| Předmět: |
Adult
Male congenital hereditary and neonatal diseases and abnormalities Cancer Research Pathology medicine.medical_specialty Malignant meningioma GTPase-activating protein Blotting Western medicine.disease_cause Proto-Oncogene Proteins p21(ras) Meningioma Tumor Cells Cultured otorhinolaryngologic diseases medicine Humans Neurofibromatosis type 2 Neurofibromatosis neoplasms Aged Neurofibromin 1 biology GTPase-Activating Proteins Proteins Middle Aged medicine.disease Precipitin Tests nervous system diseases Oncology ras GTPase-Activating Proteins Cancer research biology.protein Female Carcinogenesis Research Article |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | Meningiomas are common, mostly benign, tumours arising from leptomeningeal cells of the meninges, which frequently contain mutations in the neurofibromatosis type 2 (NF2) gene. In this study, we analysed a protein product of the neurofibromatosis type 1 (NF1) gene, neurofibromin, in human established leptomeningeal cells LTAg2B, in 17 sporadic meningiomas and in a meningioma from a patient affected by NF2. The expression level of neurofibromin was determined by immunoblotting and immunoprecipitation with anti-neurofibromin antibodies. The functional status of neurofibromin was analysed through its ability to stimulate the intrinsic GTPase activity of p21 ras. In the cytosolic extracts of four sporadic meningiomas and in the NF2-related meningioma, the expression level and the GTPase stimulatory activity of neurofibromin were drastically reduced compared with the level present in the human brain, human established leptomeningeal cells LTAg2B and the remaining 13 meningiomas. Our results suggest that neurofibromin is expressed in leptomeningeal cells LTAg2B and in most meningiomas, i.e. tumours derived from these cells. The reduced expression and GTPase stimulatory activity of neurofibromin was found in about 23% of meningiomas and in the single NF2-related meningioma analysed. These results suggest that decreased levels of neurofibromin in these tumours may contribute to their tumorigenesis. Images Figure 1 Figure 2 Figure 3 |
| Databáze: | OpenAIRE |
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