Intensification of p-coumaric acid heterologous production using extractive biphasic fermentation
| Autor: | Clémentine Fojcik, Nabila Imatoukene, Blandine Godon, Jeanne Combes, Fanny Brunissen, Michel Lopez, Julien Couvreur, Florent Allais |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Environmental Engineering 4-Vinylphenol Coumaric Acids Decarboxylation Bioengineering Saccharomyces cerevisiae 010501 environmental sciences 01 natural sciences p-Coumaric acid chemistry.chemical_compound 010608 biotechnology Waste Management and Disposal 0105 earth and related environmental sciences Chromatography Strain (chemistry) Renewable Energy Sustainability and the Environment Chemistry Extraction (chemistry) General Medicine Metabolism Oleyl alcohol Culture Media Fermentation Propionates |
| Zdroj: | Bioresource technology. 337 |
| ISSN: | 1873-2976 |
| Popis: | p-coumaric acid (p-CA) can be produced from D-glucose by an engineered S. cerevisiae strain. p-CA has antimicrobial properties and retro-inhibition activity. Moreover, p-CA is a hydrophobic compound, limiting its accumulation in fermentation broth. To overcome these issues all at once, a liquid–liquid extraction in-situ product recovery process using oleyl alcohol as extractant has been implemented in order to continuously extract p-CA from the broth. Media and pH impacts on strain metabolism were assessed, highlighting p-CA decarboxylase endogenous activity. Biphasic fermentations allowed an increase in p-CA respiratory production rates at both pH assessed (13.65 and 9.45 mg L−1.h−1 at pH 6 and 4.5, respectively) compared to control ones (10.5 and 7.5 mg L−1.h−1 at pH 6 and 4.5, respectively). Biphasic fermentation effects on p-CA decarboxylation were studied showing that continuous removal of p-CA decreased its decarboxylation into 4-vinylphenol at pH 4.5 (57 mg L−1 in biphasic fermentation vs 173 mg L−1 in control one). |
| Databáze: | OpenAIRE |
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