Calcium-dependent regulation of membrane ion permeability in a cell line derived from the equine sweat gland epithelium
| Autor: | Douglas L. Bovell, John D. Pediani, Soma Rakhit, Wing-Hung Ko, S M Wilson, James A. Nichol |
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| Rok vydání: | 1995 |
| Předmět: |
Anions
4-Acetamido-4'-isothiocyanatostilbene-2 2'-disulfonic Acid Cell Membrane Permeability Membrane permeability Ionophore chemistry.chemical_element Calcium Biology Epithelium Cell Line Iodine Radioisotopes chemistry.chemical_compound Adenosine Triphosphate Animals Channel blocker Horses Bumetanide Ion Transport Valinomycin Ionomycin General Medicine Potassium channel Sweat Glands chemistry Biochemistry Barium Biophysics Efflux Cotransporter Rubidium Radioisotopes |
| Zdroj: | Comparative Biochemistry and Physiology Part A: Physiology. 111:215-221 |
| ISSN: | 0300-9629 |
| Popis: | We measured the rates of 125 I − and 86 Rb + efflux from preloaded, cultured equine sweat gland cells. The calcium ionophore ionomycin increased the efflux of both isotopes. Anion efflux was unaffected by Ba 2+ , but this cation inhibited 86 Rb + -efflux, suggesting that [Ca 2+ ] i -activated potassium channels were present. Activation of these channels was not, however, important for the efflux of anions. We measured 125 I − efflux from valinomycin-depolarised cells in which anion cotransport was inhibited. Changes in 125 I − efflux reflect changes in anion permeability under these conditions, and ionomycin caused a clear permeability increase that was abolished by the anion channel blocker diphenylamine-2-carboxylate. ATP and UTP increased the efflux of both isotopes, suggesting that type P 2U purine receptors allow these nucleotides to regulate membrane permeability. |
| Databáze: | OpenAIRE |
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