Partial acid-hydrolysis of TEMPO-oxidized arabinoxylans generates arabinoxylan-structure resembling oligosaccharides
| Autor: | Edwin J. Bakx, Carolina O. Pandeirada, Henk A. Schols, Yvonne Westphal, Sofia Speranza, Hans Gerd Janssen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Wax
Polymers and Plastics Food Chemistry Stereochemistry Organic Chemistry Partial acid-hydrolysis Xylose Degree of polymerization Partial acid hydrolysis Organische Chemie TEMPO-oxidation chemistry.chemical_compound chemistry visual_art Arabinoxylan Levensmiddelenchemie Materials Chemistry visual_art.visual_art_medium UHPLC-PGC-MS VLAG |
| Zdroj: | Carbohydrate Polymers, 276 Carbohydrate Polymers 276 (2022) |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2021.118795 |
| Popis: | Arabinoxylans (AXs) display biological activities that depend on their chemical structures. To structurally characterize and distinguish AXs using a non-enzymatic approach, various TEMPO-oxidized AXs were partially acid-hydrolysed to obtain diagnostic oligosaccharides (OS). Arabinurono-xylo-oligomer alditols (AUXOS-A) with degree of polymerization 2–5, comprising one and two arabinuronic acid (AraA) substituents were identified in the UHPLC-PGC–MS profiles of three TEMPO-oxidized AXs, namely wheat (ox-WAX), partially-debranched WAX (ox-pD-WAX), and rye (ox-RAX). Characterization of these AUXOS-A highlighted that single-substitution of the Xyl unit preferably occurs at position O-3 for these samples, and that ox-WAX has both more single substituted and more double-substituted xylose residues in its backbone than the other AXs. Characteristic UHPLC-PGC–MS OS profiles, differing in OS abundance and composition, were obtained for each AX. Thus, partial acid-hydrolysis of TEMPO-oxidized AXs with analysis of the released OS by UHPLC-PGC-MS is a promising novel non-enzymatic approach to distinguish AXs and obtain insights into their structures. |
| Databáze: | OpenAIRE |
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