Identification of Poliovirus Isolates with Fluorescent Antibody
| Autor: | Gloria W. Ajello, S. S. Kalter, M. H. Hatch |
|---|---|
| Rok vydání: | 1961 |
| Předmět: |
Antiserum
Poliovirus Fluorescent Antibody Technique Biology medicine.disease_cause Virology General Biochemistry Genetics and Molecular Biology Enterovirus C Human Staining Microbiology Trypsinization chemistry.chemical_compound Tissue culture chemistry medicine biology.protein Humans Typing Fluorescein Antibody Poliomyelitis |
| Zdroj: | Experimental Biology and Medicine. 107:1-4 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-107-26516 |
| Popis: | Isolation and identification of enteroviruses from clinical specimens by routine methods generally require 2 or more weeks. Fluorescent antibody procedures, which have been applied to rapid diagnosis of influenza by Liu (1) and to demonstration of several viruses in tissue culture(2,3), also appear to offer the possibility of more rapid identification of enteroviruses isolated from clinical material. This report presents data concerning use of fluorescent antibody for identification of poliovirus isolates. A preliminary report of some of these data has already been made(4).Materials and methods. Hyperimmune monkey poliovirus typing sera prepared by Wenner et al.(5) were used for preparation of fluorescent antibodies. Trypsinized rhesus monkey kidney cells were employed for isolation of viruses. Buffered saline consisted of 0.14 M NaCl, 0.01 M NaH2PO4 · H2O, with the final pH adjusted to 7.0-7.2 by addition of 40% NaOH solution.Globulins were precipitated from monkey antisera by 50% saturation with (NH4)2S... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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