Culturing Mouse Cardiac Valves in the Miniature Tissue Culture System
| Autor: | Boudewijn P.T. Kruithof, Vincian Gaussin, Samuel C. Lieber, Marianna Kruithof-de Julio, Marie-José Goumans |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Aortic valve
mitral valve medicine.medical_specialty heart valve biology General Chemical Engineering Bioengineering Biology General Biochemistry Genetics and Molecular Biology Tissue Culture Techniques Miniature Tissue Culture System Tissue culture Mice In vivo Internal medicine Mitral valve Cardiac valve medicine Animals cardiovascular diseases Heart valve ex vivo flow model mouse General Immunology and Microbiology General Neuroscience Heart valve disease Heart Valves aortic valve In vitro Cell biology medicine.anatomical_structure Issue 104 cardiovascular system Cardiology Ex vivo |
| Zdroj: | Journal of Visualized Experiments ISSUE=104;TITLE=Journal of Visualized Experiments |
| Popis: | Heart valve disease is a major burden in the Western world and no effective treatment is available. This is mainly due to a lack of knowledge of the molecular, cellular and mechanical mechanisms underlying the maintenance and/or loss of the valvular structure. Current models used to study valvular biology include in vitro cultures of valvular endothelial and interstitial cells. Although, in vitro culturing models provide both cellular and molecular mechanisms, the mechanisms involved in the 3D-organization of the valve remain unclear. While in vivo models have provided insight into the molecular mechanisms underlying valvular development, insight into adult valvular biology is still elusive. In order to be able to study the regulation of the valvular 3D-organization on tissue, cellular and molecular levels, we have developed the Miniature Tissue Culture System. In this ex vivo flow model the mitral or the aortic valve is cultured in its natural position in the heart. The natural configuration and composition of the leaflet are maintained allowing the most natural response of the valvular cells to stimuli. The valves remain viable and are responsive to changing environmental conditions. This MTCS may provide advantages on studying questions including but not limited to, how does the 3D organization affect valvular biology, what factors affect 3D organization of the valve, and which network of signaling pathways regulates the 3D organization of the valve. |
| Databáze: | OpenAIRE |
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