Bidirectional membrane molecule transfer between dendritic and T cells
| Autor: | Jim Xiang, Xiaochu Wu, Zhenmin Ye, Terence Moyana, Yangdou Wei, Chaoke Tang, Yongqing Liu, Tianpei He |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
CD4-Positive T-Lymphocytes
Ovalbumin Endosome T-Lymphocytes Green Fluorescent Proteins Biophysics Endosomes Biochemistry Cell Line Humans Cytotoxic T cell IL-2 receptor CD40 Antigens Antigen-presenting cell Molecular Biology Microscopy Confocal CD40 biology T-cell receptor hemic and immune systems Dendritic Cells Cell Biology Dendritic cell Flow Cytometry CD11c Antigen Cell biology Phenotype B7-1 Antigen biology.protein Lysosomes CD80 |
| Zdroj: | Biochemical and Biophysical Research Communications. 359:202-208 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2007.05.099 |
| Popis: | The acquisition of dendritic cell (DC) molecules by T cells has been previously reported. However, it remains unclear whether the transfer is only mono- or bidirectional. In this study, we incubated CMFDA-labeled ovalbumin (OVA)-pulsed DC2.4 (DC2.4(OVA)) cells with Dil-labeled OT II CD4(+) T cells and analyzed the potential bidirectional molecule transfer. We also assessed the distribution of internalized membrane using two engineered DC2.4/Ia(b)GFP and MF4/TCRCFP DC lines. Our findings showed that membrane molecule transfer is bidirectional. CD4(+) T cells acquired Ia(b), CD11c, CD40, and CD80 from DC2.4(OVA) cells, and conversely DC2.4(OVA) cells took up CD4, CD25, CD69, and T cell receptor from T cells. The internalized molecules acquired by T cells and DCs mostly localized in endosomes and lysosomes, respectively. Taken together, this study demonstrated a novel phenomenon of bidirectional membrane molecule transfer between DCs and T cells. |
| Databáze: | OpenAIRE |
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