Biological properties of Beet soil-borne mosaic virus and Beet necrotic yellow vein virus cDNA clones produced by isothermal in vitro recombination: Insights for reassortant appearance
| Autor: | Claudio Ratti, Edgar Maiss, Katja R. Richert-Pöggeler, Marlene Laufer, Hamza Mohammad, Sebastian Liebe, Mark Varrelmann, Mattia Dall'Ara, David Gilmer |
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| Přispěvatelé: | Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Laufer, Marlene, Mohammad, Hamza, Maiss, Edgar, Richert-Pöggeler, Katja, Dall'Ara, Mattia, Ratti, Claudio, Gilmer, David, Liebe, Sebastian, Varrelmann, Mark |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Gene Expression Regulation Viral Gibson assembly DNA Complementary viruses Reassortment Nicotiana benthamiana Infectious cDNA full-length clone Benyvirus Polymyxa betae Plant Viruses 03 medical and health sciences Mosaic Viruses Complementary DNA Virology Beet necrotic yellow vein virus [SDV.BV]Life Sciences [q-bio]/Vegetal Biology RNA Viruses Cloning Molecular Beet necrotic yellow vein viru Genomic organization Plant Diseases Mosaic virus biology food and beverages Beet soil-borne mosaic viru biology.organism_classification 3. Good health Plant Leaves 030104 developmental biology Benyviridae RNA Viral Beta vulgaris Reassortant Viruses |
| Zdroj: | Virology Virology, Elsevier, 2018, 518, pp.25-33 |
| ISSN: | 1096-0341 0042-6822 |
| Popis: | Two members of the Benyviridae family and genus Benyvirus, Beet soil-borne mosaic virus (BSBMV) and Beet necrotic yellow vein virus (BNYVV), possess identical genome organization, host range and high sequence similarity; they infect Beta vulgaris with variable symptom expression. In the US, mixed infections are described with limited information about viral interactions. Vectors suitable for agroinoculation of all genome components of both viruses were constructed by isothermal in vitro recombination. All 35S promoter-driven cDNA clones allowed production of recombinant viruses competent for Nicotiana benthamiana and Beta macrocarpa systemic infection and Polymyxa betae transmission and were compared to available BNYVV B-type clone. BNYVV and BSBMV RNA1 + 2 reassortants were viable and spread long-distance in N. benthamiana with symptoms dependent on the BNYVV type. Small genomic RNAs were exchangeable and systemically infected B. macrocarpa. These infectious clones represent a powerful tool for the identification of specific molecular host-pathogen determinants. |
| Databáze: | OpenAIRE |
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